Tumor microenvironmental genomic alterations in juvenile nasopharyngeal angiofibroma
Article first published online: 16 AUG 2011
Copyright © 2011 Wiley Periodicals, Inc.
Head & Neck
Volume 34, Issue 4, pages 485–492, April 2012
How to Cite
Silveira, S. M., Domingues, M. A. C., Butugan, O., Brentani, M. M. and Rogatto, S. R. (2012), Tumor microenvironmental genomic alterations in juvenile nasopharyngeal angiofibroma. Head Neck, 34: 485–492. doi: 10.1002/hed.21767
- Issue published online: 3 MAR 2012
- Article first published online: 16 AUG 2011
- Manuscript Accepted: 24 JAN 2011
- Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil
- juvenile nasopharyngeal angiofibromas;
- quantitative real-time RT-PCR;
- chromosomal imbalances;
- high-resolution comparative genomic hybridization;
- tumor microenvironment
To better characterize the pathophysiology of juvenile nasopharyngeal angiofibroma (JNA), endothelial and stromal cells were evaluated by genomic imbalances in association with transcript expression levels of genes mapped on these altered regions.
High-resolution comparative genomic hybridization (HR-CGH) was used in laser-captured endothelial and stromal cells from 9 JNAs. Ten genes were evaluated by quantitative real-timereverse transcription polymerase chain reaction (qRT-PCR) in 15 cases.
Although gains were more frequently detected in endothelial cells, 57% of chromosomal alterations were common by both components. Gene expression analyses revealed a positive correlation between endothelial and stromal components for ASPM, CDH1, CTNNB1, FGF18, and SUPT16H. A significant difference was found for FGF18 and AURKB overexpression in stromal cells and AR down-expression in endothelial cells.
A similar pattern of gene expression and chromosomal imbalances in both exponents would suggest a common mechanism of functional regulation. AURKB, FGF18, and SUPT16H were identified as potential molecular markers in JNA. © 2011 Wiley Periodicals, Inc. Head Neck, 2012