Effects of KRAS mutation and polymorphism on the risk and prognosis of oral squamous cell carcinoma

Authors

  • Wen-Yi Wang PhD,

    1. Section of Basic Medicine, Department of Nursing, Hung Kuang University, Taichung, Taiwan
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    • Wen-Yi Wang and Yi-Chih Chien contributed equally to this work.

  • Yi-Chih Chien PhD,

    1. Department of Biology, National Changhua University of Education, Changhua, Taiwan
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    • Wen-Yi Wang and Yi-Chih Chien contributed equally to this work.

  • Yong-Kie Wong BDS, MS,

    1. Department of Oral and Maxillofacial Surgery, Taichung Veterans General Hospital, Taichung, Taiwan
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  • Yan-Liang Lin MS,

    1. Department of Biology, National Changhua University of Education, Changhua, Taiwan
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  • Jin-Ching Lin MD, PhD

    Corresponding author
    1. Department of Radiation Oncology, Taichung Veterans General Hospital, Taichung, Taiwan
    2. Department of Medicine, China Medical University, Taichung, Taiwan
    3. Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei; Taiwan
    • Department of Radiation Oncology, Taichung Veterans General Hospital, Taichung, Taiwan
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Abstract

Background

Mutations or single nucleotide polymorphism (SNP) of relevant genes may affect the risk and prognosis of malignancies. The purpose of this study was to analyze whether the KRAS polymorphisms and mutations can be useful prognostic or risk markers in oral squamous cell carcinoma (OSCC).

Methods

DNA was extracted from tumor tissues of 47 patients with OSCC and blood cells of 84 normal controls and subjected to sequencing for the KRAS.

Results

No mutation in the KRAS was found in 47 OSCC samples. However, 2 polymorphisms (rs1137282 and rs712) were detected. Individuals with KRAS SNP rs712 genotypes of G/T or T/T have a reduced risk for OSCC than those with genotype G/G (hazard ratio [HR], 0.26; 95% confidence interval [CI], 0.10–0.60; p = .004). The overall survival between different SNPs were not statistically significant (p = .147 for rs1137282 and p = .202 for rs712).

Conclusion

These data demonstrate a role for rs712 polymorphism of the KRAS in susceptibility of OSCC. © 2011 Wiley Periodicals, Inc. Head Neck, 2012

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