Case Report

Multimodal nonlinear microscopic investigations on head and neck squamous cell carcinoma: Toward intraoperative imaging

  1. Tobias Meyer Dipl.-Phys.1,
  2. Orlando Guntinas-Lichius MD2,
  3. Ferdinand von Eggeling PhD3,
  4. Günther Ernst MD3,
  5. Denis Akimov PhD1,
  6. Michael Schmitt PhD1,
  7. Benjamin Dietzek PhD1,4,
  8. Jürgen Popp PhD1,4,*

Article first published online: 18 SEP 2012

DOI: 10.1002/hed.23139

Additional Information

How to Cite

Meyer, T., Guntinas-Lichius, O., von Eggeling, F., Ernst, G., Akimov, D., Schmitt, M., Dietzek, B., Popp, J. (2012), Multimodal nonlinear microscopic investigations on head and neck squamous cell carcinoma: Toward intraoperative imaging. Head Neck. doi: 10.1002/hed.23139

Author Information

  1. 1

    Institute of Photonic Technology, Jena, Germany

  2. 2

    Department of Otorhinolaryngology, University Hospital, Jena, Germany

  3. 3

    Institute of Human Genetics, Core Unit Chip Application, University Hospital, Jena, Germany

  4. 4

    Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Jena, Germany

*Institute of Photonic Technology, Jena, Germany

Publication History

  1. Article first published online: 18 SEP 2012
  2. Manuscript Accepted: 10 JUL 2012

Funded by

  • European Union via the Europäischer Fonds für Regionale Entwicklung (EFRE)
  • Thüringer Ministerium für Bildung Wissenschaft und Kultur (TMBWK). Grant Numbers: B714-07037, B578-06001, 14.90 HWP
  • the European Network of Excellence P4L (Photonics4Life)
  • the German Ministry for Science and Education (BMBF) MediCARS. Grant Number: FKZ: 13N10774

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Keywords:

  • nonlinear microscopy;
  • head and neck cancer imaging;
  • SHG;
  • CARS;
  • TPEF

Abstract

Background

Prognosis and appropriate treatment of head and neck squamous cell carcinoma (HNSCC) depend on the tumor type routinely derived by invasive histopathology. A promising noninvasive alternative is nonlinear optical imaging, which is capable of in vivo tissue visualization for tumor typing and grading.

Methods and Results

Thin tissue sections from 3 patients aged 56 to 60 years presenting advanced carcinoma of the hypopharynx, larynx, and left tonsil were investigated by coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and 2 photon excited fluorescence (TPEF) to study the morphochemistry of the tissues. Morphologic alterations of prognostic significance, such as cell density, nuclear to cytoplasm ratio, and keratinization as well as the underlying compositional changes during malignant transformation were determined, such as the distributions of lipids, collagen, and autofluorophors.

Conclusions

Nonlinear imaging provides a noninvasive optical biopsy of the epithelial layer comparable to staining microscopy. By integration into an operational microscope, routine screening of suspicious lesions and surgical guidance can be realized. © 2012 Wiley Periodicals, Inc. Head Neck, 2012

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