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Overexpression of EphB4, EphrinB2, and epidermal growth factor receptor in papillary thyroid carcinoma: A pilot study

Authors

  • Giriraj K. Sharma MD, MS,

    1. Department of Otolaryngology – Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California
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  • Vaninder K. Dhillon MD,

    1. Department of Otolaryngology – Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California
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  • Rizwan Masood PhD,

    1. Department of Otolaryngology – Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California
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  • Dennis R. Maceri MD

    Corresponding author
    1. Department of Otolaryngology – Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California
    • Corresponding author: D. R. Maceri, Department of Otolaryngology – Head and Neck Surgery, Keck School of Medicine of University of Southern California, 1520 San Pablo Street, Suite 4600, Los Angeles, CA 90033. E-mail: maceri@usc.edu

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  • This work was presented as a poster at the 2013 annual meeting of the American Academy of Otolaryngology – Head and Neck Surgery Foundation, Vancouver, British Columbia, Canada, September 29 – October 2, 2013.

Abstract

Background

The purpose of this study was to examine the differential expression of EphB4, EphrinB2, and epidermal growth factor receptor (EGFR) genes in papillary thyroid carcinoma (PTC) and evaluate their association with lymph node metastasis.

Methods

EphB4, EphrinB2, and EGFR expression in 21 matched tumors and surrounding normal thyroid tissues were evaluated by complementary DNA (cDNA) microarray, Western blot, and immunohistochemistry (IHC).

Results

We noted a statistically significant overexpression of EphB4, EphrinB2, and EGFR in tumor versus normal tissue based on cDNA microarray, Western blot, and IHC analysis. EphB4 and EphrinB2 overexpression were significantly associated with the presence of lymph node disease.

Conclusion

Overexpression of EphB4, EphrinB2, and EGFR are associated with PTC, whereas EphB4 and EphrinB2 overexpression are associated with lymph node metastases. These genes may be potential biomarkers for identification of subclinical lymph node involvement in PTC and potential small-molecule targets for pharmacotherapy research. © 2014 Wiley Periodicals, Inc. Head Neck 37: 964–969, 2015

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