A portion of this material was presented at the International Congress of Cell Biology, Berlin, Germany, August, 1980.
Collagen Production by Rat Hepatocytes and Sinusoidal Cells in Primary Monolayer Culture†
Article first published online: 21 SEP 2007
Copyright © 1982 American Association for the Study of Liver Diseases
Volume 2, Issue 1, pages 13S–18S, January/February 1982
How to Cite
Tseng, S. C. G., Lee, P. C., Ells, P. F., Bissell, D. M., Smuckler, E. A. and Stern, R. (1982), Collagen Production by Rat Hepatocytes and Sinusoidal Cells in Primary Monolayer Culture. Hepatology, 2: 13S–18S. doi: 10.1002/hep.1840020104
- Issue published online: 21 SEP 2007
- Article first published online: 21 SEP 2007
- Manuscript Accepted: 5 OCT 1981
- Manuscript Received: 23 JUN 1981
- National Cancer Institute. Grant Number: CA 25179
- University of California
- USPHS. Grant Numbers: CA-21141, P50-AM 18520, AM 21642, AM 07007
The cellular sources of collagen in normal rat liver have been examined. Hepatocytes and nonparenchymal (sinusoidal) cells were isolated and established in primary monolayer culture. These cells were incubated with radiolabeled proline in the presence of L-ascorbate and β-amino-propionitrile. Nondialyzable material was prepared from the cell layer and the medium from each type of culture. The level of collagen accumulation was determined by measuring labeled hydroxyproline and sensitivity to purified bacterial collagenase. In hepatocytes, collagen represented 0.2% of both secreted and cell-associated labeled protein. In sinusoidal cells, collagen was 3.2% of secreted and 1.1% of cell-associated proteins. The total secreted labeled collagen, expressed per microgram of DNA, was similar in hepatocytes and sinusoidal cells. However cell-associated collagen in hepatocyte culture was approximately 10-fold that present in sinusoidal cells. These findings indicate that, while collagen formation is a relatively important function of sinusoidal cells, in normal liver the contribution of hepatocytes to total hepatic collagen accumulation may be substantial.