Mr. Sanders is currently a graduate fellow in the Department of Pathology, St. Louis University School of Medicine.
Rosette Inhibitory Factor: T-Lymphocyte Subpopulation Specificity and Potential Immunoregulatory Role in Hepatitis B Virus Infection†
Article first published online: 21 SEP 2007
Copyright © 1982 American Association for the Study of Liver Diseases
Volume 2, Issue 5, pages 547S–552S, September/October 1982
How to Cite
Sanders, G. and Perrillo, R. P. (1982), Rosette Inhibitory Factor: T-Lymphocyte Subpopulation Specificity and Potential Immunoregulatory Role in Hepatitis B Virus Infection. Hepatology, 2: 547S–552S. doi: 10.1002/hep.1840020506
This work was presented in part at the 32nd Annual Meeting of the American Association for the Study of Liver Diseases, Chicago, Illinois, November, 1981.
- Issue published online: 21 SEP 2007
- Article first published online: 21 SEP 2007
- Manuscript Accepted: 12 MAY 1982
- Manuscript Received: 20 JAN 1982
- Veterans Administration Medical Research Program 821
We have observed the disappearance of rosette inhibitory factor (RIF) from the serum of 19 patients with acute hepatitis B virus infection. This occurred at a time coinciding with the detection of anti-HBs. In addition, levels of RIF activity were significantly greater (p < 0.001) in 35 HBsAg carriers who lacked anti-HBs when compared to 15 carriers who regularly demonstrated this antibody. In all instances, RIF effect was partial affecting some, but not all, T-lymphocytes from forming erythrocyte rosettes.
To define if RIF exhibits an effect on specific T-lymphocyte subpopulations, lymphocytes from healthy donors were separated into TM (helper), TG (suppressor), and To (null) subpopulations by an immunoglobulin-ox-cell rosette depletion method. The effect of RIF on erythrocyte rosette formation and Fc-receptor expression in these subpopulations was assessed. TG-lymphocytes were found to be refractory to RIF-mediated suppression of erythrocyte rosette formation while TM-lymphocytes demonstrated an enhanced sensitivity to RIF. Incubation of TG- and To-lymphocytes, potential TM-precursor cells, with RIF resulted in a decreased expression of new IgM-Fc receptors.
In order to determine if any functional significance could be derived from these findings, the effect of RIF on in vitro immunoglobulin secretion was tested. Using pokeweed mitogen-stimulated mononuclear cell cultures, purified RIF-low density lipoprotein was shown to suppress IgM, IgG, and IgA secretion by 75.3, 74.3, and 59.3%, respectively.
These data are consistent with the hypothesis that RIF is a potential immunoregulatory protein which could contribute to the lack of anti-HBs noted during the acute phase of hepatitis B and in the majority of HBsAg carriers.