Transplantation of Woodchuck Hepatocellular Carcinoma in Nude Mice

Authors

  • Kenichi Kobayashi,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Kenichi Fukuoka,

    Corresponding author
    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    • Kenichi Kobayashi, M.D., The First Department of Internal Medicine, School of Medicine, Kanazawa University, 13–1 Takara-machi, Kanazawa, Japan.
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  • Fumiaki Matsushita,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Hideo Morimoto,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Yoshinobu Hinoue,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Hideo Honjo,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Nobuyoshi Tanaka,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Tatsuho Sugimoto,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Yasuhiro Kato,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Nobu Hattori,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Shigeharu Ueda,

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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  • Shiro Kato

    1. The First Department of Internal Medicine School of Medicine Kanazawa University 13–1 Takara-machi, Kanazawa, Japan and Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
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Abstract

Woodchuck hepatocellular carcinoma has been successfully transplanted into nude (athymic) mice. The morphology of heterotransplanted tumor is similar to that of naturally occurring hepatocellular carcinoma before transplantation. The growth rate of transplanted tumor was very slow compared with those of other transplanted tumors. During the first month, only two tumors appeared. However, definitive tumor growth was noted in 6 of 20 nude mice about 3 months later. Seventeen of 20 nude mice exhibited sustained tumor growth after 6 months. The woodchuck hepatocellular carcinoma in nude mice provides an in vivo model for the study of oncogenesis of human hepatocellular carcinoma related to hepatitis B virus.

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