This study was presented at the American Gastroenterology Association meeting, May, 1982 in Chicago, Illinois.
Ultrastructural Studies of Fibroblasts Transfected with Hepatitis B Virus DNA†
Article first published online: 24 JUL 2008
Copyright © 1984 American Association for the Study of Liver Diseases
Volume 4, Issue 1, pages 84–89, January-February 1984
How to Cite
Aoki, N., Gerber, M. A., Thung, S. N., Chen, M.-L., Christman, J. K., Price, P. M., Flordellis, C. S. and Acs, G. (1984), Ultrastructural Studies of Fibroblasts Transfected with Hepatitis B Virus DNA. Hepatology, 4: 84–89. doi: 10.1002/hep.1840040115
- Issue published online: 24 JUL 2008
- Article first published online: 24 JUL 2008
- Manuscript Accepted: 25 JUL 1983
- Manuscript Received: 20 JUL 1982
- National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases. Grant Number: Grants 30689 and 30854
Cultured 3T3 mouse fibroblasts transfected with cloned hepatitis B virus genome and DNA coding for methotrexate-resistant dihydrofolate reductase, produce and secrete significant amounts of hepatitis B surface antigen (HBsAg). Ultrastructural morphometry revealed that fibroblasts transfected with hepatitis B virus DNA contained significantly more lysosomes than did fibroblasts transfected with the gene coding for methotrexate resistance or normal fibroblasts. Although abundant HBsAg was found in the cytoplasm of transfected fibroblasts by immunologic methods, HBsAg particles were not detected by electron microscopy. Immunoelectron microscopy localized HBsAg to the nuclear envelope, rough endoplasmic reticulum, and endoplasmic cisternae. These findings suggest that the transfected cells produce mainly nonparticulate HBsAg or that they have a defect in intracisternal packaging of HBsAg into particles.