Ultrastructural Studies of Fibroblasts Transfected with Hepatitis B Virus DNA

Authors

  • Naoto Aoki,

    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
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  • Michael A. Gerber,

    Corresponding author
    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
    • Michael A. Gerber, M.D., Department of Pathology, The Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York 10029.
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  • Swan N. Thung,

    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
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  • Mei-Ling Chen,

    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
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  • Judith K. Christman,

    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
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  • Peter M. Price,

    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
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  • Christos S. Flordellis,

    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
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  • George Acs

    1. The Lillian and Henry M. Stratton-Hans Popper Department of Pathology and Departments of Biochemistry and Pediatrics, and The Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
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  • This study was presented at the American Gastroenterology Association meeting, May, 1982 in Chicago, Illinois.

Abstract

Cultured 3T3 mouse fibroblasts transfected with cloned hepatitis B virus genome and DNA coding for methotrexate-resistant dihydrofolate reductase, produce and secrete significant amounts of hepatitis B surface antigen (HBsAg). Ultrastructural morphometry revealed that fibroblasts transfected with hepatitis B virus DNA contained significantly more lysosomes than did fibroblasts transfected with the gene coding for methotrexate resistance or normal fibroblasts. Although abundant HBsAg was found in the cytoplasm of transfected fibroblasts by immunologic methods, HBsAg particles were not detected by electron microscopy. Immunoelectron microscopy localized HBsAg to the nuclear envelope, rough endoplasmic reticulum, and endoplasmic cisternae. These findings suggest that the transfected cells produce mainly nonparticulate HBsAg or that they have a defect in intracisternal packaging of HBsAg into particles.

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