Prolonged Maintenance of Active Cytochrome P-450 in Adult Rat Hepatocytes Co-Cultured with Another Liver Cell Type

Authors

  • Jean-Marc Begue,

    1. Unité de Recherches Hépatologiques, U-49 de l-INSERM, Hǒpital Pontchaillou, 35011 Rennes Cédex, France
    Current affiliation:
    1. Laboratoire de Physiologie, Faculté de Médecine, 35013 Rennes Cedex, France
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  • Christiane Guguen-Guillouzo,

    1. Unité de Recherches Hépatologiques, U-49 de l-INSERM, Hǒpital Pontchaillou, 35011 Rennes Cédex, France
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  • Nicole Pasdeloup,

    1. Unité de Recherches Hépatologiques, U-49 de l-INSERM, Hǒpital Pontchaillou, 35011 Rennes Cédex, France
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  • Andre Guillouzo

    Corresponding author
    1. Unité de Recherches Hépatologiques, U-49 de l-INSERM, Hǒpital Pontchaillou, 35011 Rennes Cédex, France
    • Dr. André Guillouzo, Unité de Recherches Hépatologiques U-49 de l' INSERM, Hǒpital Pontchaillou, 35011 Rennes Cedex, France.
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Abstract

The cytochrome P-450 content promptly fell in adult rat hepatocytes cultured in conventional conditions whereas no obvious change was detected over a 10-day period when these cells were co-cultured with another rat liver epithelial cell type. A concomitant maintenance of the amino-pyrine N-demethylase activity and a high aflatoxin B1-induced cytotoxicity was observed, and addition of phenobarbital to the culture medium produced about a 2-fold increase in cytochrome P-450 level. These results indicate that when placed in co-culture, adult hepatocytes remain differentiated for several days and suggest that such a co-culture system is an appropriate model for pharmacotoxicological studies.

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