Rat Liver Regeneration After 90% Partial Hepatectomy

Authors

  • Johannes Gaub,

    Corresponding author
    1. Division of Hepatology, Department of Medicine, Rigshospitalet, 9, Blegdamsvej, DK-2100, Copenhagen, Denmark
    • Johannes Gaub, M.D., Ph.D., Department of Epidemic Diseases M, Rigshospitalet, Tagensvej, DK 2200 Copenhagen N, Denmark.
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  • Johan Iversen

    1. Division of Hepatology, Department of Medicine, Rigshospitalet, 9, Blegdamsvej, DK-2100, Copenhagen, Denmark
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Abstract

In previous studies, 90% partial hepatectomy in the rat was invariably accompanied by 100% mortality within 40 hr. This paper describes a technique by which 90% of the liver mass can be removed with only 14% mortality, provided that rats have free access to glucose-containing drinking water. Measurements of total liver DNA, [3H]thymidine labeling index and mitotic index suggest rapid cell proliferation, commensurate with a powerful regenerative stimulus. In the presence of normoglycemia, peripheral insulin and glucagon concentrations were elevated to levels normally observed in the portal vein.

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