HLA-DR Antigens in HBsAg-Positive Chronic Active Liver Disease with and without Associated Delta Infection

Authors

  • Barbara Forzani,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Giovanni C. Actis,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Giorgio Verme,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Antonio Amoroso,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Iolanda Borelli,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Emilio S. Curtoni,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Maria Grazia Rumi,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Antonio Picciotto,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Giovanni Marinucci,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Maria Antonietta Freni,

    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
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  • Mario Rizzetto

    Corresponding author
    1. Division of Gastroenterology, Molinette, and Institute of Medical Genetics of the University, CNR Immunogenetic and Histocompatibility Center, Torino, Italy; 3rd Medical Clinic, University of Milano, Milan, Italy; Chair of Gastroenterology, University of Genova, Genova, Italy; Laboratory of Immunology, S. Giacomo Hospital Rome, Italy; and 1st Medical Clinic, University of Messina, Messina, Italy
    • Mario Rizzetto, M.D., Division of Gastroenterology, Ospedale, Molinette, Corso Bramante 88,10126, Torino, Italy.
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Abstract

The A, B, C and DR locus specificities of the human leukocyte antigens system (HLA) were determined in 45 delta-positive and 44 delta-negative Italian patients, all with HBsAg-positive chronic active liver disease; controls were 526 healthy Italian blood donors matched for age, sex and geographical origin. HLA-A, B, C gene frequencies were not significantly changed. In delta-positive patients, the frequencies of the DR locus specificities were: DR2, 37.8%; DR3, 20%; DR4, 11.1%. In the delta-negative patients, the frequencies were: DR2, 13.6%; DR3, 36.4%; DR4, 0%. Control frequencies were: DR2, 19.4%; DR3, 17.1%; DR4, 18.5%. The corrected p values of the differences between controls and delta-positive patients were: DR2, pc = 0.046; DR3, pc = NS (not significant); DR4, pc = NS. The corrected p values of the differences between controls and delta-negative patients were: DR2, pc = NS; DR3, pc = 0.03; DR4, pc = 0.002. These findings show that: (a) DR3, a genetic marker of autoimmunity, might assist the establishment of chronic HBsAg liver disease in the absence of delta superinfection; (b) DR2 is linked with failure to clear the delta agent, and (c) DR4 may protect from virus B persistence. Identification of adventitious factors such as delta may help uncover a subgroup of HBsAg carriers who are genetically predisposed to develop chronic liver disease.

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