Liver Orcein Stain and Viral DNA in Duck Hepatitis B Virus Infection in Chinese Ducks and Experimentally Infected Japanese Ducklings

Authors

  • Junko Mori,

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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  • Masao Omata,

    Corresponding author
    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
    • Masao Omata, M.D., First Department of Medicine, Chiba University School of Medicine, Chiba, Japan 280.
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  • Osamu Yokosuka,

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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  • Fumio Imazeki,

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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  • Yoshimi Ito,

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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  • Katsuo Uchiumi,

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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  • Yasuhisa Matsuyama,

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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  • Ye Weifa,

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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  • Kunio Okuda

    1. First Department of Internal Medicine, Chiba University School of Medicine, Chiba, Japan and Department of Medicine, Bethune Medical College, Changchun, China
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Abstract

Liver sections were stained with orcein, and duck hepatitis B virus was identified in sera and livers by the hybridization technique in 106 ducks (44 Chinese ducks, 15 Japanese ducks and 47 Japanese ducklings). Orcein-positive hepatocytes were found in 18 of 38 (47%) duck hepatitis B virus DNA seropositive ducks, and only in 3 of 68 (4%) seronegative ducks. The three ducks were all from a heavily infected flock in southern China.

Serial analyses of viral DNA by Southern blot and spot hybridizations in experimentally infected Japanese ducklings revealed a dissociation or a time gap between the amount of viral DNA in serum and the emergence of orcein positive hepatocytes. Orcein-positive hepatocytes were generally associated with prolonged presence of viral infection for at least 4 to 6 months. These findings support the clinical hypothesis that the presence of orcein-positive hepatocytes indicates persistent rather than acute infection.

Since orcein-positive hepatocytes have been seen in infection with hepatitis B, woodchuck hepatitis, ground squirrel and duck hepatitis B viruses, accumulation of orcein-positive material in liver cells may be one of the common properties these viruses share. This stain may be utilized for screening new hepatitis B virus-like viruses.

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