Endocytosis and binding of asialoorosomucoid by hepatocytes from rats with jejunoileal bypass

Authors

  • Nathalie Serbource-Goguel/Seta,

    1. Laboratoire de Biochimie, U.E.R. de Pharmacie, 92290
    2. Chatenay-Malabry, France and Laboratoire d'Anatomo-Pathologic and Service de Chirurgie Digestive, C.H.U. Cǒte de Nacre, 14040 Caen Cedex, France
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  • Béatrice Borel,

    1. Laboratoire de Biochimie, U.E.R. de Pharmacie, 92290
    2. Chatenay-Malabry, France and Laboratoire d'Anatomo-Pathologic and Service de Chirurgie Digestive, C.H.U. Cǒte de Nacre, 14040 Caen Cedex, France
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  • Michèle Dodeur,

    1. Laboratoire de Biochimie, U.E.R. de Pharmacie, 92290
    2. Chatenay-Malabry, France and Laboratoire d'Anatomo-Pathologic and Service de Chirurgie Digestive, C.H.U. Cǒte de Nacre, 14040 Caen Cedex, France
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  • Pierre Scarmato,

    1. Laboratoire de Biochimie, U.E.R. de Pharmacie, 92290
    2. Chatenay-Malabry, France and Laboratoire d'Anatomo-Pathologic and Service de Chirurgie Digestive, C.H.U. Cǒte de Nacre, 14040 Caen Cedex, France
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  • Benoit Bourel,

    1. Laboratoire de Biochimie, U.E.R. de Pharmacie, 92290
    2. Chatenay-Malabry, France and Laboratoire d'Anatomo-Pathologic and Service de Chirurgie Digestive, C.H.U. Cǒte de Nacre, 14040 Caen Cedex, France
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  • Jeanne Feger,

    1. Laboratoire de Biochimie, U.E.R. de Pharmacie, 92290
    2. Chatenay-Malabry, France and Laboratoire d'Anatomo-Pathologic and Service de Chirurgie Digestive, C.H.U. Cǒte de Nacre, 14040 Caen Cedex, France
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  • Dr. Geneviève Durand

    Corresponding author
    1. Laboratoire de Biochimie, U.E.R. de Pharmacie, 92290
    2. Chatenay-Malabry, France and Laboratoire d'Anatomo-Pathologic and Service de Chirurgie Digestive, C.H.U. Cǒte de Nacre, 14040 Caen Cedex, France
    • Laboratoire de Biochimie, U.E.R. de Pharmacie, Rue J. B. Clément, 92290 Chatenay-Malabry, France
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Abstract

Rats with Jejunoileal bypass were used to study the biological activity of the hepatic binding protein. Hepatocytes were prepared 11 weeks after surgical procedure, and presence of asialoo-rosomucoid in serum has been determined.

Compared to control rat hepatocytes, endocytosis of [3H]asialoorosomucoid by bypassed rat hepatocytes was decreased by about 60%. This was due to a decreased number of total and surface receptors. In most sera, an accumulation of asialoorosomucoid was found. A reduction of protein synthesis or turnover could be considered.

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