Functional properties of isolated hepatocytes from ethanol-treated rat liver

Authors

  • Amador Schüller M. D.,

    Corresponding author
    1. Medical Pathology Cathedra III, Clinical Hospital and Department of Biochemistry, Faculty of Chemistry, Universidad Complutense, Madrid, Spain
    • Hospital Clinico de San Carlos de la Facultad de Medicina, Madrid 3, Spain
    Search for more papers by this author
  • Jorge Moscat,

    1. Medical Pathology Cathedra III, Clinical Hospital and Department of Biochemistry, Faculty of Chemistry, Universidad Complutense, Madrid, Spain
    Search for more papers by this author
  • Emilio Diez,

    1. Medical Pathology Cathedra III, Clinical Hospital and Department of Biochemistry, Faculty of Chemistry, Universidad Complutense, Madrid, Spain
    Search for more papers by this author
  • Jose C. Fernandez-Checa,

    1. Medical Pathology Cathedra III, Clinical Hospital and Department of Biochemistry, Faculty of Chemistry, Universidad Complutense, Madrid, Spain
    Search for more papers by this author
  • Francisco G. Gavilanes,

    1. Medical Pathology Cathedra III, Clinical Hospital and Department of Biochemistry, Faculty of Chemistry, Universidad Complutense, Madrid, Spain
    Search for more papers by this author
  • Angel M. Municio

    1. Medical Pathology Cathedra III, Clinical Hospital and Department of Biochemistry, Faculty of Chemistry, Universidad Complutense, Madrid, Spain
    Search for more papers by this author

Abstract

Gluconeogenesis and palmitate incorporation into triacylglycerols and phosphatidylcholine were measured in isolated hepatocytes from control and ethanol-treated rats. Basal gluconeogenesis and its hormonal response decreased in hepatocytes from ethanol-treated animals; palmitate incorporation into triacylglycerols increased. In ethanol-treated rat liver, 45Ca2+ uptake and methylating capacity were reduced, and the hormonal response exhibited differences in binding parameters of insulin.

Ancillary