Dr. Theilmann was the recipient of a fellowship from the Deutsche Forschungsgemeinschaft.
Reduced transport of bilirubin and asialoorosomucoid in regenerating rat liver is a microtubule-independent event
Article first published online: 5 DEC 2005
Copyright © 1985 American Association for the Study of Liver Diseases
Volume 5, Issue 5, pages 798–804, September/October 1985
How to Cite
Stollman, Y. R., Theilmann, L., Stockert, R. J. and Wolkoff, A. W. (1985), Reduced transport of bilirubin and asialoorosomucoid in regenerating rat liver is a microtubule-independent event. Hepatology, 5: 798–804. doi: 10.1002/hep.1840050515
- Issue published online: 5 DEC 2005
- Article first published online: 5 DEC 2005
- Manuscript Accepted: 7 JUN 1985
- Manuscript Received: 9 JAN 1985
- NIH. Grant Numbers: AM 23026, AM 32419, AM 17702, AM 32972
In previous studies, we found that uptake of bilirubin and asialoorosomucoid is depressed in regenerating rat liver. To determine what role the hepatic cytoskeleton plays in this modulation of uptake, animals were treated with colchicine, an inhibitor of microtubular polymerization. Normal unoperated rats or rats following two-thirds hepatectomy or sham surgery were injected with colchicine, lumicolchicine (50 μg per 100 gm of body weight, i.p.) or normal saline. Lumicolchicine, an analog of colchicine, has no effect on microtubules and was used as a control. At 12 hr after surgery, sham-operated and unoperated animals received a second equal dose. Partially hepatectomized animals received one-third the initial dose. At 24 hr after surgery, livers were perfused in situ and single-pass multiple indicator dilution studies were performed. Colchicine as compared to lumicolchicine pretreatment reduced apparent influx of bilirubin and asialoorosomucoid in regenerating liver by 50% but had no effect in liver from normal or sham-operated rats. Analysis of indicator dilution curves revealed that reduced influx in colchicine-treated liver was attributable to an increased vascular volume of distribution. These results suggest that microtubules may play a role in maintenance of normal hepatic vascular architecture during regeneration. Lack of effect of colchicine on modulation of bilirubin and asialoorosomucoid uptake during regeneration suggests that other, as yet unknown, factors result in down-regulation of the specific hepatocellular transport systems for these two ligands.