Measurement of fibrosis in needle liver biopsies: Evaluation of a colorimetric method

Authors

  • Wladimiro Jimenez,

    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
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  • Albert Parés,

    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
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  • Joan Caballería M.D.,

    Corresponding author
    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
    • Liver Unit, Hospital Clínic i Provincial, Casanova 143, 08036 Barcelona, Spain
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  • Daniel Heredia,

    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
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  • Meguel Bruguera,

    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
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  • Miguel Torres,

    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
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  • Marcos Rojkind,

    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
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  • Joan Rodés

    1. Liver Unit, Hospital Clínic i Provincial, University of Barcelona, Barcelona, Spain
    2. Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del INP, México DF, México
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Abstract

Collagen content was measured in 38 needle liver biopsies (8 steatosis, 8 chronic hepatitis, 7 fibrosis and 15 cirrhosis) by a new colorimetric method based on the selective capacity of Sirius red and Fast green to bind to collagen and noncollagenous proteins, respectively. The values were compared with those obtained after determination of the degree of fibrosis by morphometry in the same tissue.

In biopsies with cirrhosis and fibrosis, there was a higher amount of collagen than in biopsies with chronic hepatitis and steatosis. Furthermore, there was a highly significant direct correlation between the collagen content measured colorimetrically and the degree of fibrosis determined morphometrically (r = 0.77, p < 0.001), suggesting that this new colorimetric method is useful in measuring the degree of fibrosis in needle liver biopsies.

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