Measurement of fibrosis in needle liver biopsies: Evaluation of a colorimetric method
Article first published online: 5 DEC 2005
Copyright © 1985 American Association for the Study of Liver Diseases
Volume 5, Issue 5, pages 815–818, September/October 1985
How to Cite
Jimenez, W., Parés, A., Caballería, J., Heredia, D., Bruguera, M., Torres, M., Rojkind, M. and Rodés, J. (1985), Measurement of fibrosis in needle liver biopsies: Evaluation of a colorimetric method. Hepatology, 5: 815–818. doi: 10.1002/hep.1840050517
- Issue published online: 5 DEC 2005
- Article first published online: 5 DEC 2005
- Manuscript Accepted: 24 APR 1985
- Manuscript Received: 28 DEC 1984
- Comisión Asesora de Investigación Científica. Grant Number: 1214/81
- Técnica of Ministerio de Educación y Ciencia
Collagen content was measured in 38 needle liver biopsies (8 steatosis, 8 chronic hepatitis, 7 fibrosis and 15 cirrhosis) by a new colorimetric method based on the selective capacity of Sirius red and Fast green to bind to collagen and noncollagenous proteins, respectively. The values were compared with those obtained after determination of the degree of fibrosis by morphometry in the same tissue.
In biopsies with cirrhosis and fibrosis, there was a higher amount of collagen than in biopsies with chronic hepatitis and steatosis. Furthermore, there was a highly significant direct correlation between the collagen content measured colorimetrically and the degree of fibrosis determined morphometrically (r = 0.77, p < 0.001), suggesting that this new colorimetric method is useful in measuring the degree of fibrosis in needle liver biopsies.