Establishment of a cell line from a woodchuck hepatocellular carcinoma

Authors

  • Masashi Unoura,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Kenichi Kobayashi M.D.,

    Corresponding author
    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
    • The First Department of Internal Medicine, School of Medicine, Kanazawa University, 13-1 Takara-Machi, Kanazawa, Japan
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  • Kenichi Fukuoka,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Fumiaki Matsushita,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Hideo Morimoto,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Tohru Oshima,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Shuichi Kaneko,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Nobu Hattori,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Seishi Murakami,

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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  • Hiroshi Yoshikawa

    1. First Department of Internal Medicine, School of Medicine, Division of Biophysics Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa, Japan
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Abstract

A new cell line derived from a woodchuck hepatocellular carcinoma serially transplanted in athymic nude mice has been established and named WH257GE10. The original tumor in the nude mouse system produces woodchuck hepatitis surface antigen and albumin. In addition, woodchuck hepatitis virus DNA is integrated into cellular DNA. Adaptation of the cells to the in vitro culture condition was completed after 15 months with the doubling time of 40 hr. The morphologic features of the cell by light microscopy are of an epithelial type. The modal chromosome number is 36 and the karyotype is mainly metacentric, similar to that observed in normal woodchuck liver cells. Ornithine and tyrosine aminotransferase activities were detected. Production of albumin was demonstrated in the cytoplasm by indirect immunofluorescence. Integration of woodchuck hepatitis virus DNA was shown by Southern blot analysis, although the secretion of woodchuck hepatitis surface antigen was not detected. This cell line provides an excellent in vitro model to study human hepatocellular carcinoma related to hepatitis B virus.

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