Liver DNA synthesis promoter activity detected in human plasma from subjects with hepatitis

Authors

  • Dr. Juan J. Díaz-Gil Ph.D.,

    Corresponding author
    1. Departments of Experimental Biochemistry and Gastroenterology, Clínica Puerta de Hierro, 28035 Madrid, Spain
    2. Department of Morphology, Facultad de Medicina, Universidad Autónoma, 28029 Madrid, Spain
    • Servicio de Bioquímica Experimental, Clínica Puerta de Hierro, San Martin de Porres, 4, 28035 Madrid, Spain
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  • Gonzalo Sánchez,

    1. Departments of Experimental Biochemistry and Gastroenterology, Clínica Puerta de Hierro, 28035 Madrid, Spain
    2. Department of Morphology, Facultad de Medicina, Universidad Autónoma, 28029 Madrid, Spain
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  • Luis Santamaría,

    1. Departments of Experimental Biochemistry and Gastroenterology, Clínica Puerta de Hierro, 28035 Madrid, Spain
    2. Department of Morphology, Facultad de Medicina, Universidad Autónoma, 28029 Madrid, Spain
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  • Carolina Trilla,

    1. Departments of Experimental Biochemistry and Gastroenterology, Clínica Puerta de Hierro, 28035 Madrid, Spain
    2. Department of Morphology, Facultad de Medicina, Universidad Autónoma, 28029 Madrid, Spain
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  • Paloma Esteban,

    1. Departments of Experimental Biochemistry and Gastroenterology, Clínica Puerta de Hierro, 28035 Madrid, Spain
    2. Department of Morphology, Facultad de Medicina, Universidad Autónoma, 28029 Madrid, Spain
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  • Pedro Escartín

    1. Departments of Experimental Biochemistry and Gastroenterology, Clínica Puerta de Hierro, 28035 Madrid, Spain
    2. Department of Morphology, Facultad de Medicina, Universidad Autónoma, 28029 Madrid, Spain
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Abstract

A liver DNA synthesis promoter activity was detected in human plasma from subjects with hepatitis. The assay procedure consisted of intraperitoneal injection into mice of aliquots of plasma, previously chromatographed on Sephadex G-25. After 24 hr, [3H]thymidine was injected and its incorporation into liver DNA measured. The increase in [3H]thymidine uptake of injected mice was not detected in those administered plasma from normal subjects (basal [3H]thymidine incorporation was that corresponding to saline-injected mouse values). At a maximal effective dose (0.3 mg protein per mouse), plasma from subjects with hepatitis increased the mitotic index of mouse liver hepatocytes; at the same dose, plasma from normal subjects had no effect. This DNA synthesis promoter activity appears to be a protein, as it is sensitive to trypsin digestion and heat.

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