Cytophotometric measurements of nuclear DNA content in hepatocellular carcinomas

Authors

  • Sow-Hsong Kuo M.D.,

    Corresponding author
    1. Departments of Clinical Pathology, Internal Medicine and Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan 10016, Republic of China
    • Department of Clinical Pathology, National Taiwan University Hospital, Taipei, Taiwan 10016, Republic of China
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  • Jin-Chuan Sheu,

    1. Departments of Clinical Pathology, Internal Medicine and Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan 10016, Republic of China
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  • Ding-Shinn Chen,

    1. Departments of Clinical Pathology, Internal Medicine and Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan 10016, Republic of China
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  • Juei-Low Sung,

    1. Departments of Clinical Pathology, Internal Medicine and Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan 10016, Republic of China
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  • Chi-Chung Lin,

    1. Departments of Clinical Pathology, Internal Medicine and Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan 10016, Republic of China
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  • Hey-Chi Hsu

    1. Departments of Clinical Pathology, Internal Medicine and Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan 10016, Republic of China
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Abstract

The nuclear DNA contents of 54 hepatocellular carcinomas were measured on cytologic smears using a scanning microdensitometer at a wavelength of 550 nm. The cellular materials were aspirated under ultrasonographic guidance and stained with modified Feulgen method. The DNA modal values, which could be defined in 51 cases, ranged from 2.2 to 9.8 ploidy, with 46.3% (25/54) in hypertetraploidy. The hepatocellular carcinomas in patients with higher serum levels of α-fetoprotein contained more hyperploid cells. The DNA modal values did not correlate with the age, HBsAg status, tumor size and histological grading of hepatocellular carcinoma. The S-phase fraction values, which could be estimated on the DNA histograms in 43 cases (79.6%), ranged from 1 to 35 (mean ± S.D. = 10.6 ± 8.1). The S-phase fraction values were higher in high grade hepatocellular carcinomas (13.4 ± 8.5) than low grade hepatocellular carcinomas (6.1 ± 4.4), p < 0.01. The mean S-phase fraction values were also significantly higher in hepatocellular carcinomas with α-fetoprotein levels of more than 100 ng per ml than those of less than 100 ng per ml (12.9 ± 8.0 and 7.8 ± 5.6, respectively). The S-phase fraction values also did not correlate with the age, HBsAg status and tumor size.

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