Establishment of a new cell line from a woodchuck hepatocellular carcinoma

Authors

  • Shin Ohnishi,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Hiromu Aoyama,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Junji Shiga,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Yuji Itai,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Takashi Moriyama,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Takashi Ishikawa,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Nobuo Sasaki,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Koshi Yamamoto,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Kaoru Koshimizu,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Shuichi Kaneko,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Seishi Murakami,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Nobu Hattori,

    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    Search for more papers by this author
  • Michio Imawari M.D.

    Corresponding author
    1. Third Department of Internal Medicine, Departments of Pathology and Radiology and Division of Animal Research, Faculty of Medicine and Department of Veterinary Surgery, Faculty of Agriculture, University of Tokyo, Tokyo 113, Japan
    2. First Department of Internal Medicine and Division of Biophysics Cancer Research Institute, School of Medicine, Kanazawa University, Kanazawa, Japan
    • The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7–3—1 Hongo, Bunkyo-ku, Tokyo 113, Japan
    Search for more papers by this author

Abstract

A new cell line derived from a woodchuck hepatitis surface antigen-positive woodchuck hepatocellular carcinoma has been established and named T3-HEP-W1. This new cell line was established directly from a primary woodchuck hepatocellular carcinoma. Adaptation of the cells to the in vitro culture condition was completed after 3 months, with the doubling time of 24 hr. The morphologic features of the cell by light microscopy were of an epithelial type. The modal chromosome number was 100. Ornithine and tyrosine aminotransferase activities were detected. Production of albumin was negative. Integration of woodchuck hepatitis virus DNA was demonstrated by Southern blot analysis, although the secretion of woodchuck hepatitis surface antigen was not detected. T3-HEP-W1 is quite different from the previously reported WH257GE10 cell line and provides another in vitro model for the study of human hepatocellular carcinoma related to hepatitis B virus.

Ancillary