Validation of viscosity measurements for canine hepatic bile

Authors

  • David D. Wilson,

    1. Department of Pharmacology, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20307-5100
    Current affiliation:
    1. Nova Pharmaceutical Corp., 5210 Eastern Avenue, Baltimore, Maryland 21224
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  • Howard S. Lowensohn Ph.D.

    Corresponding author
    1. Department of Pharmacology, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20307-5100
    • Department of Pharmacology, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20307–5100
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Abstract

This study developed the validated standards for a known viscosity method by obtaining consistent viscosity measurements with defined precision, reproducibility and sensitivity limits for canine hepatic bile. Size 75 Cannon-Manning semimicro viscometers yielded the most precise viscosity measurements. With the development of proper handling methods for fresh bile samples, a precision defined by a coefficient of variation of ≤ ± 0.3% was obtained. To maintain viscometer constancy, the viscometers must be cleaned with chromic acid after each bile test and pass reproducibility tests using defined distilled water viscosity tests.

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