Localization of adrenergic and neuropeptide tyrosine-containing nerves in the mammalian liver

Authors

  • Alastair D. Burt M.D.,

    Corresponding author
    1. University Department of Pathology, Western Infirmary, Glasgow, Scotland
    2. Department of Cell Bio1ogy and Histology, Free University of Brussels (VUB), 1090 Brussels-Jette, Belgium
    3. Department of Histochemistry, Royal Postgraduate Medical School, London, England
    • University Department of Pathology. Western Infirmary, Glasgow G11 6NT, Scotland
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    • Dr. Burt was supported by a Peel Trust Travelling Research Fellowship and by the Kinnear Brown Trust.

  • Dina Tiniakos,

    1. University Department of Pathology, Western Infirmary, Glasgow, Scotland
    2. Department of Cell Bio1ogy and Histology, Free University of Brussels (VUB), 1090 Brussels-Jette, Belgium
    3. Department of Histochemistry, Royal Postgraduate Medical School, London, England
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  • Roderick N. M. MacSween,

    1. University Department of Pathology, Western Infirmary, Glasgow, Scotland
    2. Department of Cell Bio1ogy and Histology, Free University of Brussels (VUB), 1090 Brussels-Jette, Belgium
    3. Department of Histochemistry, Royal Postgraduate Medical School, London, England
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  • Maureen R. Griffiths,

    1. University Department of Pathology, Western Infirmary, Glasgow, Scotland
    2. Department of Cell Bio1ogy and Histology, Free University of Brussels (VUB), 1090 Brussels-Jette, Belgium
    3. Department of Histochemistry, Royal Postgraduate Medical School, London, England
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  • Eddie Wisse,

    1. University Department of Pathology, Western Infirmary, Glasgow, Scotland
    2. Department of Cell Bio1ogy and Histology, Free University of Brussels (VUB), 1090 Brussels-Jette, Belgium
    3. Department of Histochemistry, Royal Postgraduate Medical School, London, England
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  • Julia M. Polak

    1. University Department of Pathology, Western Infirmary, Glasgow, Scotland
    2. Department of Cell Bio1ogy and Histology, Free University of Brussels (VUB), 1090 Brussels-Jette, Belgium
    3. Department of Histochemistry, Royal Postgraduate Medical School, London, England
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Abstract

The distribution of adrenergic nerves in guinea pig and rat liver was studied by the immunolocalization of fibers containing tyrosine hydroxylase and dopamine β-hydroxylase, enzymes involved in the synthesis of catecholamines. In both species, adrenergic fibers were identified within portal tracts, often in close proximity hepatic artery branches. In guinea pig liver, but not liver, abundant intraacinar fibers were identified; fibers were also seen within the walls of terminal hepatic vein radicles and larger hepatic veins. The presence of peptidergic nerves containing the regulatory peptide neuropeptide tyrosine and the C-flanking peptide CPON was investigated by indirect immunofluorescence. The distribution of these nerves was similar to that of tyrosine hydroxylase- and dopamine β-hydroxylase-positive nerves and showed the same species difference. The effector sympathetic nature of tyrosine hydroxylase- and neuropeptide tyrosine-positive fibers rat liver was confirmed by chemical denervation studies using 6-hydroxydopamine.

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