was the recipient of a Medical Research Council of Canada fellowship.
Increased prooxidant action of hepatic cytosolic low-molecular-weight iron in experimental iron overload
Article first published online: 6 DEC 2005
Copyright © 1990 American Association for the Study of Liver Diseases
Volume 11, Issue 6, pages 1038–1043, June 1990
How to Cite
Britton, R. S., Ferrali, M., Magiera, C. J., Recknagel, R. O. and Bacon, B. R. (1990), Increased prooxidant action of hepatic cytosolic low-molecular-weight iron in experimental iron overload. Hepatology, 11: 1038–1043. doi: 10.1002/hep.1840110620
- Issue published online: 6 DEC 2005
- Article first published online: 6 DEC 2005
- Manuscript Accepted: 16 JAN 1990
- Manuscript Received: 21 AUG 1989
- U.S. Public Health Service. Grant Numbers: R23 DK 35469, RO1 ES 01821
In the iron-loaded liver there may be an increase in the putative intracellular transit pool of iron, components of which could be catalytically active in stimulating lipid peroxidation. To study the levels of low-molecular-weight, catalytically active iron in the liver, cytosolic ultrafiltrates were tested in an assay containing rat liver microsomes and NADPH. Malondialdehyde production was used as an index of lipid peroxidation. This assay system was sensitive enough to detect 0.25 μmol/L ferrous iron; progressive but nonlinear increases in malondialdehyde were produced as the iron concentration was increased to 5 μmol/L. Ultrafiltrates from hepatic cytosol of iron-loaded rats had greater prooxidant action than did those from controls. When added to the assay, deferoxamine, an iron chelator, completely suppressed the prooxidant action of hepatic ultrafiltrates, showing that this activity is iron-dependent. Deferoxamine administered intraperitoneally to control animals at a dose of 1 gm/kg completely inhibited the prooxidant effect of hepatic ultrafiltrates prepared from rats killed after 1, 2 and 3 hr. Partial inhibition was observed at 4 hr; by 6 hr the inhibitory effect of deferoxamine was completely lost. Administration of deferoxamine (1 gm/kg intraperitoneally, 1 hr before killing) completely inhibited the prooxidant action of hepatic ultrafiltrates in moderately iron-loaded rats and control but had no protective effect in heavily iron-loaded rats. These results support the concept that iron overload results in an increase in a hepatic cytosolic pool of low-molecular-weight iron that is catalytically active in stimulating lipid peroxidation. This pool can be chelated transiently in vivo by deferoxamine in moderate, but not heavy, iron overload.(HEPATOLOGY 1990;11:1038-1043.).