This paper is dedicated to Professor Dr. W. Gerok in honor of his sixty-fifth birthday.
Article first published online: 5 DEC 2005
Copyright © 1991 American Association for the Study of Liver Diseases
Volume 13, Issue 3, pages 562–566, March 1991
How to Cite
Gerbes, A. L., Kollenda, M. C., Vollmar, A. M., Reichen, J., Vakil, N. and Scarborough, R. M. (1991), Altered density of glomerular binding sites for atrial natriuretic factor in bile duct–ligated rats with ascites. Hepatology, 13: 562–566. doi: 10.1002/hep.1840130326
Parts of this study were presented at the Fortieth Annual Meeting of the AASLD, October 28–31, 1989, Chicago, Illinois, and at the Twenty-fourth Annual Meeting of the EASL, August 30 - September 2, 1989, Munich, Germany. This work contains data that are part of a doctoral thesis by M.C. Kollenda at the Ludwig-Maximilianus-Universität, München.
- Issue published online: 5 DEC 2005
- Article first published online: 5 DEC 2005
- Manuscript Accepted: 20 SEP 1990
- Manuscript Received: 1 MAR 1990
- Deutsche Forschungsgemeinschaft. Grant Number: GE 576
- SNF. Grant Number: 32-9365.87
The renal response to atrial natriuretic factor is blunted in cirrhosis with ascites. This might be due to alterations of renal receptors for atrial natriuretic factor. Therefore density and affinity of glomerular atrial natriuretic factor binding sites of bile duct–ligated rats with ascites (n = 10) and of sham-operated controls (n = 10) were determined. Glomerular atrial natriuretic factor binding sites were identified to be of the B-(“biologically active”) and C-(“clearance”) receptor type. Discrimination and quantitative determination of B and C receptors for atrial natriuretic factor were achieved by displacement experiments with atrial natriuretic factor(99–126) or des(18–22)atrial natriuretic factor(4–23), an analogue binding to C receptors only. Density of total glomerular atrial natriuretic factor binding sites was significantly increased in bile duct-ligated rats (3,518 ± 864 vs. 1,648 ± 358 fmol/mg protein; p < 0.05). This was due to a significant increase of C-receptor density (3,460 ± 866 vs. 1,486 ± 363 fmol/mg protein; p < 0.05), whereas density of B receptors was not significantly different in bile duct–ligated rats (58 ± 11 vs. 162 ± 63 fmol/mg protein). Affinity of atrial natriuretic factor to its glomerular binding sites did not differ significantly between both groups. These data suggest that an altered glomerular atrial natriuretic factor receptor density could be involved in the renal resistance to atrial natriuretic factor in cirrhosis with ascites. (HEPATOLOGY 1991;13:562–566.)