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Immunohistochemical demonstration of pancreatic α-amylase and trypsin in intrahepatic bile ducts and peribiliary glands

Authors

  • Tadashi Terada M.D.,

    Corresponding author
    1. Second Department of Pathology, Kanazawa University School of Medicine, Kanazawa 920, Japan
    • Second Department of Pathology, Kanazawa University School of Medicine, Kanazawa 920, Japan
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  • Yasuni Nakanuma

    1. Second Department of Pathology, Kanazawa University School of Medicine, Kanazawa 920, Japan
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Abstract

Epithelia of intrahepatic bile ducts and peribiliary glands were immunohistochemically examined for pancreatic α-amylase and trypsin in 54 normal autopsied livers. α-Amylase was evaluated with a polyclonal antibody, and trypsin was assayed with both polyclonal and monoclonal antibodies. α-Amylase was observed in large ducts, septal ducts and peribiliary glands in most livers and was seen in interlobular ducts in seven (13%) livers. Trypsin immunoreactivity with the polyclonal antibody was observed in peribiliary glands in 21 (39%) livers; it was absent in intrahepatic bile ducts in all but one liver. Trypsin immunoreactivity with the monoclonal antibody was present in large ducts, septal ducts and peribiliary glands in about 70% of the livers and was seen in interlobular ducts in two (4%) livers. Bile ductules were always negative for the two antigens. Some epithelia of peribiliary glands positive for both α-amylase and trypsin histologically resembled pancreatic acinar cells. α-Amylase and trypsin immunoreactivities of intrahepatic biliary epithelia and pancreatic aninar cells were eliminated by absorption of primary antibodies by α-amylase or trypsin, suggesting the specificities of the immunoreactivities. These data suggest that epithelia of intrahepatic large ducts, septal ducts and peribiliary glands contain pancreatic α-amylase in most livers and that they contain trypsin in about 70% of livers. α-Amylase and trypsin may be secreted into intrahepatic bile duct lumens, thereby exerting important effects on the physiology of the intrahepatic biliary tree and hepatic bile. (HEPATOLOGY 1991;14:1129–1135.)

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