Anti—pre-S responses and viral clearance in chronic hepatitis B virus infection

Authors

  • Agata Budkowska Ph.D.,

    Corresponding author
    1. Microbial Immunology Unit and World Health Organization Collaborating Center for Reference and Research on Viral Hepatitis, Institut Pasteur 75724 Paris Cédex 15, France
    • Immunologic Microbienne, Institut Pasteur, 75724 Paris, Cedex 15, France
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  • Pascal Dubreuil,

    1. Microbiology and Hepatogastroenterology Department, Hǒpital Antoine Béclère, 92141 Clamart, France
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  • Thierry Poynard,

    1. Microbiology and Hepatogastroenterology Department, Hǒpital Antoine Béclère, 92141 Clamart, France
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  • Patrick Marcellin,

    1. Hepatology Department, Hǒpital Beaujon, 92118 Clichy, France
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  • Marie-Anne Loriot,

    1. Hepatology Department, Hǒpital Beaujon, 92118 Clichy, France
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  • Patrick Maillard,

    1. Microbial Immunology Unit and World Health Organization Collaborating Center for Reference and Research on Viral Hepatitis, Institut Pasteur 75724 Paris Cédex 15, France
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  • Jacques Pillot

    1. Microbial Immunology Unit and World Health Organization Collaborating Center for Reference and Research on Viral Hepatitis, Institut Pasteur 75724 Paris Cédex 15, France
    2. Microbiology and Hepatogastroenterology Department, Hǒpital Antoine Béclère, 92141 Clamart, France
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Abstract

Serial sera were collected prospectively during the clinical course of 13 HBsAg carriers with chronic liver disease and analyzed for ALT levels, pre-S1 and pre-S2 antigens and corresponding antibodies and other serological hepatitis B virus markers. In five patients, anti—pre-S1 and anti—pre-S2 antibodies became detectable in multiple serum samples, whereas in eight patients anti—pre-S was never detected or only appeared transiently during the follow-up. The first pattern was associated with normalization of ALT levels and undetectable pre-S antigens and viral DNA by the polymerase chain reaction assay at final follow-up. HBsAg clearance occurred in two of the five patients. The second pattern was one of persistence of HBsAg and pre-S antigens, associated with the presence of serum HBV DNA detectable by spot hybridization or polymerase chain reaction regardless of clinical outcome. These findings demonstrate the occurrence of anti—pre-S antibodies in chronic hepatitis B virus—induced liver disease and associate anti—pre-S appearance with the clearance of hepatitis B virus from serum. (HEPATOLOGY 1992;15:26–31).

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