Improved serodiagnosis of non-A, non-B hepatitis by an assay detecting antibody to hepatitis C virus core antigen
Article first published online: 6 DEC 2005
Copyright © 1992 Wiley Subscription Services, Inc.
Volume 15, Issue 3, pages 391–394, March 1992
How to Cite
Katayama, T., Mazda, T., Kikuchi, S., Harada, S., Matsuura, Y., Chiba, J., Ohba, H., Saito, I. and Miyamura, T. (1992), Improved serodiagnosis of non-A, non-B hepatitis by an assay detecting antibody to hepatitis C virus core antigen. Hepatology, 15: 391–394. doi: 10.1002/hep.1840150306
- Issue published online: 6 DEC 2005
- Article first published online: 6 DEC 2005
- Manuscript Accepted: 11 OCT 1991
- Manuscript Received: 15 JUL 1991
- Ministry of Health and Welfare, and the Ministry of Education, Science and Culture, Japan
We examined sequential serum samples from 12 patients with well-characterized posttransfusion non-A, non-B hepatitis who had an acute, resolving self-limited type of clinical course for the presence of antibody to the hepatitis C virus nucleocapsid (core) protein (p22) expressed by a recombinant baculovirus. These sera were simultaneously examined for antibody to the hepatitis C virus nonstructural protein (C 100-3) that is presently used for blood screening worldwide. In three patients, both anti-p22 and anti-C 100-3 antibodies were detected, but anti-p22 was detected much earlier. In four patients, only anti-p22 was detected. Two other patients were considered to be hepatitis C virus carriers who had been already infected with hepatitis C virus. In one patient, only anti-C 100-3 was detected, and it was transient. In two patients, neither antibody was detected. Anti-p22 was detected in at least one of eight samples of transfused blood. Of the nine samples of donated blood that were positive for anti-p22, only four were positive for anti-C 100-3. This new assay detecting the antibody to the p22 protein is thus useful for the serodiagnosis of non-A, non-B hepatitis in the acute phase and for blood screening. (Hepatology 1992;15:391–394).