Localization of cytochrome P-450 gene expression in normal and diseased human liver by in situ hybridization of wax-embedded archival material

Authors

  • Colin N. A. Palmer,

    1. Department of Biochemistry, Queen Mary and Westfield College, University of London, London E1 4NS
    Current affiliation:
    1. Department of Basic and Clinical Research, Scripps Clinic and Research Foundation, La Jolla, CA 92037
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  • Philip J. Coates,

    1. Department of Biochemistry, Queen Mary and Westfield College, University of London, London E1 4NS
    Current affiliation:
    1. Department of Histopathology, United Medical and Dental Schools, St. Thomas' Hospital, London SE1 7EH, UK
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  • Susan E. Davies,

    1. Department of Histopathology, St. Bartholomew's Hospital, London EC1A 7BE
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  • Elizabeth A. Shephard,

    1. Department of Biochemistry and Molecular Biology, University College London, London WC1E 6BT, United Kingdom
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  • Ian R. Phillips Ph.D.

    Corresponding author
    1. Department of Biochemistry, Queen Mary and Westfield College, University of London, London E1 4NS
    • Department of Biochemistry, Queen Mary and Westfield College, University of London, Mile End Road, London E1 4NS, UK
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Abstract

The localization of the expression of several cytochrome P-450 genes in normal and diseased human liver was investigated by in situ hybridization of formalin-fixed, paraffin wax–embedded archival tissue samples with 35S-labeled antisense RNA probes. The results demonstrated that genes coding for members of the cytochrome P-450 3A subfamily (CYP3A) were preferentially expressed in hepatocytes in acinar zone 3 (the centrilobular region), whereas genes coding for CYP1A2, CYP2A, 2B and 2C were expressed uniformly throughout the liver acinus. In cirrhotic livers, CYP2A and 2B genes (and to a lesser extent, CYP3A genes) were highly expressed in isolated hepatocytes located at the junction of parenchyma with fibrous septa. The cause and significance of the position-dependent expression of specific cytochrome P-450 genes in normal and diseased human liver are discussed. (HEPATOLOGY 1992;16:682–687.)

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