Increased neutrophil function induced by bile duct ligation in a rat model

Authors

  • Rachel Levy Ph.D.,

    Corresponding author
    1. Infectious Diseases Laboratory, Clinical Biochemistry Unit, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
    • Infectious Diseases Laboratory, Clinical Biochemistry Unit, Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
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  • Francisc Schlaeffer,

    1. Infectious Diseases Laboratory, Clinical Biochemistry Unit, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
    2. Department of Internal Medicine, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
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  • Arie Keynan,

    1. Department of Internal Medicine, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
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  • Ofra Nagauker,

    1. Infectious Diseases Laboratory, Clinical Biochemistry Unit, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
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  • Arie Yaari,

    1. Department of Internal Medicine, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
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  • Emanuel Sikuler

    1. Liver Diseases Unit, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
    2. Department of Internal Medicine, Soroka Medical Center of Kupat-Holim and Faculty of Health Sciences, Ben Gurion University, Beer-Sheva 84105, Israel
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Abstract

Neutrophil function was studied in rats with common bile duct ligation. Superoxide production stimulated by phorhol myristate acetate, opsonized zymosan or formyl-methionyl-leucyl-phenylalanine; phagocytosis; and chemotaxis were significantly greater in neutrophils from rats with common bile duct ligation than in sham-operated control rats. Enhanced neutrophil activity was observed within 12 hr of bile duct ligation; it remained increased during the 15-day study. Preincubation of neutrophils from control rats with sera of rats with common bile duct ligation did not increase superoxide generation. This suggests that the high superoxide production observed in neutrophils of rats with common bile duct ligation was not an immediate effect of the serum. Neutrophils of rats with portal vein ligation exhibited normal activity, indicating that portal systemic shunting per se is not the underlying mechanism for increased activity. The elevated levels of AST and alkaline phosphatase, indicating liver damage, that appeared within 12 hr of bile duct ligation correlated with the increased superoxide generation. (HEPATOLOGY 1993;17:908–914.)

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