Proliferative and cytotoxic T-cell clones recognize endogenously synthesized HBcAG in an asymptomatic HBsAg carrier

Authors

  • Pei-Yun Shu,

    1. Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei 112
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  • Chungming Chang,

    1. Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei 112
    2. Department of Medical Research, Veterans General Hospital, Taipei 112
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  • Lih-Hwa Hwan,

    1. Hepatitis Research Center, National Taiwan University Hospital, Taipei 107, Taiwan, Republic of China
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  • Cheng-Po Hu

    Corresponding author
    1. Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei 112
    2. Department of Medical Research, Veterans General Hospital, Taipei 112
    • Department of Medical Research, Veterans General Hospital, Taipei 112, Taiwan, Republic of China
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Abstract

The characterization of immune responses to hepatitis B virus is crucial for the understanding of hepatitis B virus–caused liver disease. However, lack of a suitable autologous effector–target cell system makes a precise study of the pathogenesis of hepatitis B difficult. In this study we established a model system by using autologous HBcAg-expressing Epstein-Barr virus–immortalized lymphoblastoid cell lines as stimulator/target cells. T-cell cultures were established by repetitive stimulation with recombinant HBcAg or autologous HBcAg-expressing lymphoblastoid cell lines. Both proliferative and cytotoxic T-cell clones were obtained from the peripheral blood of an asymptomatic HBsAg carrier. Clones T12 (CD8+) and T2B (CD4+) were cytotoxic clones specific against autologous lymphoblastoid cell lines expressing endogenously synthesized HBcAg, whereas five CD4+ T-cell clones proliferated in response to lymphoblastoid cell lines incubated with exogenous recombinant HBcAg and autologous HBcAg-expressing lymphoblastoid cell lines. These results indicate that autologous HBcAg-expressing lymphoblastoid cell lines are appropriate stimulator/target cells for the study of HBcAg-specific T lymphocytes. By using this approach, we have demonstrated that both proliferative and cytotoxic T lymphocytes recognizing endogenously synthesized HBcAg are induced during chronic hepatitis B virus infection. (HEPATOLOGY 1993;18:275–283).

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