Production of chemotactic factor, interleukin-8, from hepatocytes exposed to ethanol

Authors

  • Yasushi Shiratori M. D.,

    Corresponding author
    1. Division of Gastroenterology, Institute of Adult Disease, Asahi Life Foundation, Tokyo 160, Japan
    2. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    • Department of Internal Medicine(II), Faculty of Medicine, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan
    Search for more papers by this author
  • Hiroshi Takada,

    1. Division of Gastroenterology, Institute of Adult Disease, Asahi Life Foundation, Tokyo 160, Japan
    2. Department of Gastroenterology and Hepatology, Dokkyo University School of Medicine, Koshigaya Hospital Saitama 343, Japan
    Search for more papers by this author
  • Yohko Hikiba,

    1. Division of Gastroenterology, Institute of Adult Disease, Asahi Life Foundation, Tokyo 160, Japan
    2. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Search for more papers by this author
  • Ryo Nakata,

    1. Division of Gastroenterology, Institute of Adult Disease, Asahi Life Foundation, Tokyo 160, Japan
    2. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Search for more papers by this author
  • Ken'Ichi Okano,

    1. Division of Gastroenterology, Institute of Adult Disease, Asahi Life Foundation, Tokyo 160, Japan
    Search for more papers by this author
  • Yutaka Komatsu,

    1. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Search for more papers by this author
  • Yasuro Niwa,

    1. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Search for more papers by this author
  • Masayuki Matsumura,

    1. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Search for more papers by this author
  • Shuichiro Shiina,

    1. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Search for more papers by this author
  • Masao Omata,

    1. Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    Search for more papers by this author
  • Kazuo Kamii

    1. Department of Gastroenterology and Hepatology, Dokkyo University School of Medicine, Koshigaya Hospital Saitama 343, Japan
    Search for more papers by this author

Abstract

In a previous paper, we demonstrated proteinous chemotactic factors released from hepatocytes exposed to ethanol. In this study, we further characterized the chemotactic factors released from ethanoltreated hepatocytes. After fractionating the conditioned medium with gel chromatography, we demonstrated chemotactic activity at molecular weights of around 20 and 40 kD. When the conditioned medium was mixed with antiserum against interleukin-8, chemotactic activity was almost completely abolished. In addition, antiserum against interleukin-8 completely reduced chemotactic activity of the 20-kD chemotactic factor but did not influence the chemotactic activity of the 40-kD chemotactic factor. After gel electrophoresis of the conditioned medium, the protein reacted with antibody against interleukin-8 was demonstrated at a molecular weight of 20 kD but was not found at a molecular weight of 40 kD. These results suggest that a major part of the proteinous chemotactic factors released from hepatocytes exposed to ethanol could be a dimer form of interleukin-8, one of the proinflammatory cytokines, possibly contributing to the pathogenesis of alcoholic hepatitis. (HEPATOLOGY 1993;18:1477–1482.)

Ancillary