DNA ploidy analysis of hepatic preneoplastic and neoplastic lesions in woodchucks experimentally infected with woodchuck hepatitis virus

Authors

  • Dr. Li-Jun Mi,

    1. Immunopathology Laboratory of the Veterans Affairs Medical Center, Bronx, New York 10468
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    • Dr. Mi is a Visiting Research Fellow from the Department of Infectious Diseases, Second Hospital of Harbin Medical College, Harbin, People's Republic of China

  • Jaygonda Patil,

    1. Immunopathology Laboratory of the Veterans Affairs Medical Center, Bronx, New York 10468
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  • William E. Hornbuckle,

    1. Department of Clinical Sciences, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853
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  • Paul J. Cote,

    1. Division of Molecular Virology and Immunology, Georgetown University School of Medicine, Rockville, Maryland 20852
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  • John L. Gerin,

    1. Division of Molecular Virology and Immunology, Georgetown University School of Medicine, Rockville, Maryland 20852
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  • Bud C. Tennant,

    1. Department of Clinical Sciences, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853
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  • Fiorenzo Paronetto M.D.

    Corresponding author
    1. Immunopathology Laboratory of the Veterans Affairs Medical Center, Bronx, New York 10468
    2. Lillian and Henry M. Stratton-Hans Popper Department of Pathology of the Mount Sinai School of Medicine of the CUNY, New York, New York 10029
    • Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, 130 West Kingsbridge Road, Bronx, NY 10468
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Abstract

We analyzed the DNA ploidy and the nuclear size of hepatocytes within hepatocellular carcinoma, putative preneoplastic (clear cell and basophilic foci) and adjacent non-neoplastic liver in 30 woodchucks neonatally infected with the woodchuck hepatitis virus. In livers from control woodchucks, in clear cell foci and in most chronic portal hepatitis, the hepatocytes were diploid, with less than 10% tetraploid cells. Aneuploid peaks were found in 50% of the livers with chronic active hepatitis, in 63% of basophilic foci and in 90% of hepatocellular carcinoma. Within the same tumor, aneuploid peaks with different DNA indices were observed frequently, indicating heterogeneity of tumor. S-phase was always elevated, indicating an increased rate of proliferation. Aneuploid cells had nuclei that were larger than those of control liver cells. In some basophilic foci and in some livers with chronic active hepatitis, abnormal DNA was demonstrated before the development of hepatocellular carcinoma, suggesting that these may be populations of hepatocytes at risk of neoplastic transformation. (Hepatology 1994;20:21–29.)

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