Viral Hepatitis

You have full text access to this OnlineOpen article

Quantitative serum HBV DNA levels during different stages of chronic hepatitis B infection

  1. Chi-Jen Chu,
  2. Munira Hussain,
  3. Anna S. F. Lok M.D.*

Article first published online: 7 MAR 2007

DOI: 10.1002/hep.1840360617

Issue

Hepatology

Hepatology

Volume 36, Issue 6, pages 1408–1415, December 2002

Additional Information

How to Cite

Chu, C.-J., Hussain, M. and Lok, A. S. F. (2002), Quantitative serum HBV DNA levels during different stages of chronic hepatitis B infection. Hepatology, 36: 1408–1415. doi: 10.1002/hep.1840360617

Author Information

  1. Department of Medicine, Division of Gastroenterology, University of Michigan Medical Center, Ann Arbor, MI

*Division of Gastroenterology, University of Michigan Medical Center, 3912 Taubman Center, Box 0362, Ann Arbor, MI 48109. fax: 734–936–7392

Publication History

  1. Issue published online: 7 MAR 2007
  2. Article first published online: 7 MAR 2007
  3. Manuscript Accepted: 11 SEP 2002
  4. Manuscript Received: 24 JUN 2002

Funded by

  • NIDDK. Grant Numbers: NO1-DK-92323, 1UO1-DK-57577, 1UO1-DK-60344

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (737K)

Reference

  • 1
    Loeb KR, Jerome KR, Goddard J, Huang ML, Cent A, Corey L. High-throughput quantitative analysis of hepatitis B virus DNA in serum using the TaqMan fluorogenic detection system. Hepatology 2000; 32: 626–629.
    Direct Link:
  • 2
    Noborg U, Gusdal A, Horal P, Lindh M. Levels of viraemia in subjects with serologic markers of past or chronic hepatitis B virus infection. Scand J Infect Dis 2000; 32: 249–252.
  • 3
    Niitsuma H, Ishii M, Miura M, Kobayashi K, Toyota T. Low level hepatitis B viremia detected by polymerase chain reaction accompanies the absence of HBe antigenemia and hepatitis in hepatitis B virus carriers. Am J Gastroenterol 1997; 92: 119–123.
  • 4
    Gerken G, Gomes J, Lampertico P, Colombo M, Rothaar T, Trippler M, Colucci G. Clinical evaluation and applications of the Amplicor HBV MonitorTM test, a quantitative HBV DNA PCR assay. J Virol Methods 1998; 74: 155–165.
  • 5
    Fujiwara K, Yokosuka O, Ehata T, Chuang WL, Imazeki F, Saisho H, Omata M. The two different states of hepatitis B virus DNA in asymptomatic carriers: HBeAg-antigen—positive versus anti-HBe—positive asymptomatic carriers. Dig Dis Sci 1998; 43: 368–376.
  • 6
    Lindh M, Horal P, Dhillon AP, Norkrans G. Hepatitis B virus DNA levels, precore mutations, genotypes and histological activity in chronic hepatitis B. J Viral Hepatitis 2000; 7: 258–267.
    Direct Link:
  • 7
    Kessler HH, Preininger S, Stelzl E, Daghofer E, Santner BI, Marth E, Lackner H, et al. Identification of different states of hepatitis B virus infection with a quantitative PCR assay. Clin Diagn Lab Immun 2000; 7: 298–300.
  • 8
    Chu CJ, Lok AS. Clinical utility of quantifying serum HBV DNA levels using PCR assays. J Hepatol 2002; 36: 549–551.
  • 9
    Chan HLY, Leung NWY, Hussain M, Wong ML, Lok ASF. Hepatitis B e antigen—negative chronic hepatitis B in Hong Kong. Hepatology 2000; 31: 763–768.
    Direct Link:
  • 10
    Jardi R, Buti M, Rodriguez-Frias F, Cortina M, Esteban R, Guardia J, Pascual C. The value of quantitative detection of HBV-DNA amplified by PCR in the study of hepatitis B infection. J Hepatol 1996; 24: 680–685.
  • 11
    Gauthier J, Bourne EJ, Lutz MW, Crowther LM, Dienstag JL, Brown NA, Condreay LD. Quantitation of hepatitis B viremia and emergence of YMDD variants in patients with chronic hepatitis B treated with lamivudine. J Infect Dis 1999; 180: 1757–1762.
  • 12
    Lok AS, Heathcote EJ, Hoofnagle JH. Management of Hepatitis B 2000, Summary of a Workshop. Gastroenterology 2001; 120: 1828–1853.
  • 13
    Martinot-Peignoux M, Boyer N, Colombat M, Akremii R, Pham BN, Castelnau C, Valla D, et al. Serum HBV DNA levels and liver histology in inactive HBsAg carriers. J Hepatol 2002; 36: 543–546.
  • 14
    Manesis EK, Papatheodoridis GV, Hadziyannis SJ. Serum HBV DNA levels in inactive hepatitis B virus carriers. Gastroenterology 2002; 122: 2092–2093.
  • 15
    Ranki M, Schatzl HM, Zachoval R, Uusi-Oukari M, Lehtovaara P. Quantification of hepatitis B virus DNA over a wide range from serum studying viral replicative activity in response to treatment and in recurrent infection. Hepatology 1995; 21: 1492–1499.
    Direct Link:
  • 16
    Noborg U, Gusdal A, Pisa EK, Hedrum A, Lindh M. Automated quantitative analysis of hepatitis B virus DNA by using the Cobas Amplicor HBV Monitor test. J Clin Microbiol 1999; 37: 2793–2797.
  • 17
    Pawlotsky JM, Bastie A, Hezode C, Lonjon I, Darthuy F, Remire J, Dhumeaux D. Routine detection and quantification of hepatitis B virus DNA in clinical laboratories: performance of three commercial assays. J Virol Methods 2000; 85: 11–21.
  • 18
    Teles SA, Martins RMB, Vanderborght B, Stuyver L, Gasper AMC, Yoshida CFT. Hepatitis B virus: genotypes and subtypes in Brazilian hemodialysis patients. Artif Organs 1999; 23: 1074–1078.
    Direct Link:
  • 19
    Grandjacques C, Pradat P, Stuyver L, Chevallier M, Chevallier P, Pichoud C, Maisonnas M, et al. Rapid detection of genotypes and mutations in the pre-core promotor and the pre-core region of hepatitis B virus genome: correlation with viral persistence and disease severity. J Hepatol 2000; 33: 430–439.
  • 20
    Chu CJ, Hussain M, Lok ASF. Hepatitis B virus genotype B is associated with earlier HBeAg seroconversion compared to hepatitis B virus genotype C. Gastroenterology 2002; 122: 1756–1762.
  • 21
    Norder H, Courouce AM, Magnius LO. Complete genomes, phylogenetic relatedness and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 1994; 198: 489–503.
  • 22
    Stuyver L, de Gendt S, van Geyt C, Zoulim F, Fried M, Schinazi RF, Rossau R. A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 2000; 81: 67–74.
  • 23
    Lok AS, Akarca U, Greene S. Mutation in the pre-core region of hepatitis B virus serve to enhance the stability of the secondary structure of the pre-genome encapsidation signal. Proc Natl Acad Sci U S A 1994; 91: 4077–4081.
  • 24
    Hadziyannis SJ, Vassilopoulos D. Hepatitis e antigen-negative chronic hepatitis B. Hepatology 2001; 34: 617–624.
    Direct Link:
Get PDF (737K)