Viral Hepatitis

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Expression profiling of liver cell lines expressing entire or parts of hepatitis C virus open reading frame

  1. Hideki Aizaki1,
  2. Takashi Harada1,†,
  3. Motoyuki Otsuka2,
  4. Naohiko Seki3,
  5. Mami Matsuda1,
  6. Yue Wei Li1,
  7. Hayato Kawakami4,
  8. Yoshiharu Matsuura5,
  9. Tatsuo Miyamura1,
  10. Tetsuro Suzuki Ph.D.1,*

Article first published online: 7 MAR 2007

DOI: 10.1002/hep.1840360620

Issue

Hepatology

Hepatology

Volume 36, Issue 6, pages 1431–1438, December 2002

Additional Information

How to Cite

Aizaki, H., Harada, T., Otsuka, M., Seki, N., Matsuda, M., Li, Y. W., Kawakami, H., Matsuura, Y., Miyamura, T. and Suzuki, T. (2002), Expression profiling of liver cell lines expressing entire or parts of hepatitis C virus open reading frame. Hepatology, 36: 1431–1438. doi: 10.1002/hep.1840360620

Author Information

  1. 1

    Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan

  2. 2

    Department of Gastroenterology, Faculty of Medicine, University of Tokyo, Tokyo, Japan

  3. 3

    Department of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan

  4. 4

    Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan

  5. 5

    Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

  1. †

    Department of Immunology, Immunobiology Research Institute, Chiron, Siena, Italy

*Department of Virology II, National Institute of Infectious Diseases, 1–23–1, Toyama, Shinjuku-ku, Tokyo, 162–8640, Japan. fax: (81) 3–5285–1161

Publication History

  1. Issue published online: 7 MAR 2007
  2. Article first published online: 7 MAR 2007
  3. Manuscript Accepted: 1 SEP 2002
  4. Manuscript Received: 2 MAY 2002

Funded by

  • Ministry of Health and Welfare and the Organization for Pharmaceutical Safety and Research, Japan

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Abstract

Although hepatitis C virus (HCV) is a causative agent of liver diseases, its mechanism of pathogenesis is still unclear, mainly because of the lack of adequate cell culture systems to support HCV infection and replication. In this report, we describe development and characterization of human hepatoma cell lines constitutively expressing entire (Hep394) or parts (Hep352, Hep3294) of the HCV open reading frame (ORF). The viral and cellular proteolytic machinery involved in the viral precursor processing was consistently functional, and processed HCV proteins were synthesized in these established cell lines. By using a cDNA microarray analysis coupled with semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), we identified 12 genes up-regulated and 4 genes down-regulated in Hep394 cells. With regard to genes related to cell growth regulation, we found up-regulation of forkhead transcription factor FREAC-1, poly (A) binding protein PABP2, and Ras suppressor Rsu-1. Another category of changes in gene expression includes MHC antigens, which play an important role in the T-cell-mediated immune reaction in the liver. In conclusion, functional genomic approaches comparing expression among the different cell lines expressing parts of the HCV genome may promote our understanding of the molecular basis of pathogenicity of HCV infection. (HEPATOLOGY2002;36:1431–1438).

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