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suppmat_assenat_fig1.tif1128KHuman hepatocytes were cultured with 100 U/mL IL-1β for different periods of time (0, 4, 8, 12, or 24 hours). Cells were then harvested in Trizol (Life Technologies, Inc) and total RNA extracted. Twenty five μg of total RNA were subjected to Northen blot (GAPDH and TAT) or RNAse protection (CAR and GR) analysis.
suppmat_assenat_fig2.tif6906KHeLa or HepG2 cell lines were cotransfected with expression plasmid for hCAR (pBSEN-hCAR) and both (CYP3A4 ER6)3-pGL3p and pRSV-bgalactosidase reporter plasmids. Cells were treated or not for 24 h with IL-1β (25 or 100 U/mL) or LPS (1 or 10 μg/mL). Cell extracts were as sayed for luciferase activity which was normalized to β-galactosidase activity. Data points represent the mean of assays performed in triplicate.
suppmat_assenat_fig3.tif4853KSchematic representation of inflammation induced- repression of CAR and CAR's target genes expression

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