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Liver Failure and Liver Disease
Article first published online: 24 MAR 2005
Copyright © 2005 American Association for the Study of Liver Diseases
Volume 41, Issue 4, pages 722–730, April 2005
How to Cite
Unitt, E., Rushbrook, S. M., Marshall, A., Davies, S., Gibbs, P., Morris, L. S., Coleman, N. and Alexander, G. J. M. (2005), Compromised lymphocytes infiltrate hepatocellular carcinoma: The role of T-regulatory cells. Hepatology, 41: 722–730. doi: 10.1002/hep.20644
See Editorial on Page 700.
Potential conflict of interest: Nothing to report.
- Issue published online: 24 MAR 2005
- Article first published online: 24 MAR 2005
- Manuscript Accepted: 18 JAN 2005
- Manuscript Received: 26 SEP 2004
- The Digestive Diseases Foundation
- The National Blood Service
- Addenbrooke's Hospital Hepatology Endowment Fund
- Beverley Read Memorial Fund
Hepatocellular carcinoma (HCC) has a poor prognosis with limited therapeutic options. We propose that local immune responses in patients with HCC are held in check by tumor-infiltrating CD4+CD25+ T-regulatory lymphocytes (Treg cells), which suppress the activity and proliferation of effector CD4+ and CD8+ T cells. The phenotype and cell cycle status of tumor-infiltrating lymphocytes (TILs) in HCC were analyzed via immunohistochemistry of sections from patients undergoing surgery for HCC and via flow cytometry of peripheral blood mononuclear cells and TILs isolated from patients with HCC. Circulating and tumor-infiltrating T-cell function and activation status were assessed via proliferation and flow cytometry. More than 96% of TILs were quiescent as measured via Mcm-2 or Ki-67 expression, while less than 10% of CD8+ T cells expressed perforin or granzyme B. CD4+CD25+ Treg cells comprised 8.7% (1.4–13.8) of TILs and always exceeded the proportion in distant nontumor tissue (2.4% [1.5–5.6]; P = .014). Treg cells isolated from HCC suppressed proliferation of autologous circulating CD4+CD25− cells and perforin expression and proliferation of autologous CD8+ T cells. The proportion of circulating Treg cells in patients with HCC was similar in healthy controls (7.2% [1.2–23.3] and 9.2% [1.6–30.2], respectively), but the proportion of circulating Treg cells that were also transforming growth factor β1+ was elevated in HCC compared with controls (55.5% [8.2–73.9] and 2.0% [0–4.9], respectively; P = .003). In conclusion, TILs are compromised and contain a subpopulation of suppressive CD4+CD25+Foxp3+ Treg cells. Functional deletion of tumor-infiltrating Treg cells could enhance tumor-specific immunotherapy. (HEPATOLOGY 2005;41:722–730.)