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Liver Biology and Pathobiology
Fibroblast activation protein increases apoptosis, cell adhesion, and migration by the LX-2 human stellate cell line†
Article first published online: 20 SEP 2005
Copyright © 2005 American Association for the Study of Liver Diseases
Volume 42, Issue 4, pages 935–945, October 2005
How to Cite
Wang, X. M., Yu, D. M. T., McCaughan, G. W. and Gorrell, M. D. (2005), Fibroblast activation protein increases apoptosis, cell adhesion, and migration by the LX-2 human stellate cell line. Hepatology, 42: 935–945. doi: 10.1002/hep.20853
Potential conflict of interest: Nothing to report.
- Issue published online: 20 SEP 2005
- Article first published online: 20 SEP 2005
- Manuscript Accepted: 12 JUL 2005
- Manuscript Received: 1 OCT 2004
- Australian National Health and Medical Research Council. Grant Numbers: 142606, 358398
This article includes Supplementary Material available at http://www.interscience.wiley.com/jpages/0270-9139/suppmat
|jws-hep.20853.fig1.tif||52K||Molecular phenotype of FAP andDPIV overexpressing 293T cells. Flow cytometry of CFP fluorescencepositive cells immunostained for CD44, integrin &#x03b2; 1 ,&#x03b2;-catenin, MMP2 and DDR1 two days after CFP, CFP-FAP (A, C, E, G,I) or DPIV-CFP (B, D, F, H, J) transfection. Cells werenon-permeabilized (i) for cell surface staining or permeabilized(ii) to stain both internal and surface molecules. Stainingintensity differences (Table 3) were calculated by reference toeach corresponding negative control antibody profile to show thatFAP or DPIV overexpression stimulated CD44, integrin &#x03b2; 1 and MMP2 expression and depressed b-catenin and DDR1 expression.All cells were first gated for CFP expression. CFP transfectedcells stained with antibody (._._._.) or control antibody(..&#8230;); FAP or DPIV transfected cells stained with antibody( ____ ) or control antibody (____).|
|jws-hep.20853.fig2.jpg||357K||(i) Molecular phenotype of LX-2 cells(A-G). Flow cytometry showing that LX-2 cells were immunopositivefor integrin &#x03b2; 1(A), integrin &#x03b1; 3(B), CXCR4(C), CXCL12 (D), &#x03b2;-catenin (E), DDR1 (F), and MMP2 (G). Antibody( ____ ). Control antibody (____). (ii) Molecularphenotype of migrating LX-2 cells (H-J). LX-2 cells above themembrane (non-migrated) and below the membrane (migrated) wereexamined following transwell assays. Expression of integrin&#x03b2; 1(H), &#x03b2;-catenin (I), and DDR1 (J). Similar data were obtained from GFP-FAP-positive cells (H-J) and GFP-positive cells(Supplemental Fig. 2). Migrated cells ( ____ ).Non-migrated cells (____). GFP, glial fibrillary protein; FAP,fibroblast activation protein.|
|jws-hep.20853.fig3.tif||3180K||Molecular phenotype of migratingLX-2 cells. Following transwell experiments, GFP fluorescencepositive cells above the membrane (non-migrated) and below themembrane (migrated) were phenotyped by flow cytometry. Moreintegrin &#x03b2; 1was on migrated than non-migrated cells(A-F). More migrated than non-migrated cells expressed &#x03b2;-catenin;about 20 % of non-migrated cells but < 10 % of migrated cellslacked detectable b-catenin expression (G-L). DDR1 (M-R) and CD44and MMP2 (not shown) were not differentially expressed. Cells werenon-permeabilized for integrin &#x03b2; 1staining orpermeabilized for &#x03b2;-catenin and DDR1 staining. Antibody staining(solid lines), control antibody (dotted line), migrated cells( ____ ), non-migrated cells (____).|
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