Supplementary material for this article can be found on the H EPATOLOGY website ( ).

jws-hep.20962.fig1.tif26987KDevelopment of GST 7-7(+) hepatocytes in rat strains treated according to the &&num;8220;resistant hepatocyte&&num;8221; model at different times after initiation with diethylnitrosamine. Data are expressed as the mean &&num;177; SD of 4 to 7 rats. Studentttest: *BN versus F344 (at leastP< .05). DENA, diethylnitrosamine; BD, basal diet; AAF, BD containing 0.02% 2-acetylaminofluorene; PH, partial hepatectomy; F344, Fisher 344; BN, Brown Norway.
jws-hep.20962.fig2.tif26833KInstantImager images of a representative experiment of DNA 32P-postlabeling with DNA extracted from control liver or liver of rats treated with diethylnitrosamine or 2-acetylaminofluorene. Quantitative analysis (right panel): mean values &&num;177; S. (n = 4) of DNA adduct levels, corrected for the radioactivity in approximately equivalent unspotted areas of the chromatograms, adjacent to the spots. Studentttest: &&num;8226;with versus without carcinogen ( P< .001); *BN versus F344 ( P= .0005). F344, Fisher 344; BN, Brown Norway; C, control; DENA, diethylnitrosamine; E-dG, ethyl-deoxyguanosine; E-dT, ethyl-deoxythymidine; AAF, 2-acetylaminofluorene; dG-AF, deoxyguanosine aminofluorene.
jws-hep.20962.fig3.tif21891KIncorporation of (A) [ 3 H]thymidine and expression of (B) P16 INK4A and (C)CDC37genes in HepG2 cells cultured 48 and 72 hours without (control) or with 100 nmol/L scramble olygonucleotide or siRNAs (A), (B), or (C). Data are expressed as the mean &&num;177; SD of 4 experiments. N target = 2 &&num;8211;&&num;916;Ct , where the &&num;916;Ct value of the sample was calculated by subtracting the average Ct value of the target gene from the average Ct value of theRNR-18gene. Studentttest: &&num;8226;siRNA versus control and scramble olygonucleotide (at least P< .05). NT, N target; siRNA, small interfering RNA.

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