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Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html )

FilenameFormatSizeDescription
jws-hep.21005.mat-mtd.doc24K Immunohistochemistry FluorescenceIn SituHybridization for Y-Chromosome and GFP staining
jws-hep.21005.fig1.TIF449KPositive and negative controls of liver immunostaining for the identification of hematopoietic cells (CD45 + ) and/or EGFP +cells.A , Liver section of a CCl 4treated and X-ray irradiated animal stained with anti-EGFP (green) and anti-mCD45 (red) antibodies;B , Liver sections of a EGFP-transgenic mouse stained as inA . Note the variegation in the intensity of the EGFP expression by the transgenic hepatocytes.

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