Potential conflict of interest: Nothing to report.
A novel function for a micro RNA: Negative regulators can do positive for the hepatitis C virus†
Article first published online: 22 FEB 2006
Copyright © 2006 American Association for the Study of Liver Diseases
Volume 43, Issue 3, pages 612–615, March 2006
How to Cite
Appel, N., Bartenschlager, R. (2006), A novel function for a micro RNA: Negative regulators can do positive for the hepatitis C virus. Hepatology, 43: 612–615. doi: 10.1002/hep.21092
- Issue published online: 22 FEB 2006
- Article first published online: 22 FEB 2006
MicroRNAs are small RNA molecules that regulate messenger RNA (mRNA) expression. MicroRNA 122 (miR-122) is specifically expressed and highly abundant in the human liver. We show that the sequestration of miR-122 in liver cells results in marked loss of autonomously replicating hepatitis C viral RNAs. A genetic interaction between miR-122 and the 5′ noncoding region of the viral genome was revealed by mutational analyses of the predicted microRNA binding site and ectopic expression of miR-122 molecules containing compensatory mutations. Studies with replication-defective RNAs suggested that miR-122 did not detectably affect mRNA translation or RNA stability. Therefore, miR-122 is likely to facilitate replication of the viral RNA, suggesting that miR-122 may present a target for antiviral intervention.