Supplementary materials for this article can be found on the H EPATOLOGY website ( ).

jws-hep.21174.fig1.TIF109KSuppl Fig 1. IL-15 pretreatment decreases NKT-derived cytokine productions in Con A-induced hepatitis.Mice were treated subcutaneously with PBS or IL-15 for 6 h, followed by administration with Con A. Serum samples were collected at various time points post Con A injection, and serum levels of IL-4 (A), IL-5 (B), IFN- (C) and TNF-&&num;x03b1; (D) were measured by ELISA. Values in panels A-D are shown as means &&num;x00b1;SEM (n=5). **, P<0.01, *, P<0.05 as comparison with Con A group. (E) For flow cytometric analysis, one hour after Con A injection, liver MNCs were prepared and examined for various intracellular cytokines production. T (CD3 + NK1.1 - ), NKT (CD3 + NK1.1 + ) and NK (CD3 - NK1.1 + ) cells were then examined for intracellular IL-4, IL-5 IFN- and TNF-&&num;x03b1; by flow cytometry after triple staining with anti-NK1.1, anti-CD3 and the indicated intracellular antibodies.
jws-hep.21174.fig2.TIF119KSuppl Fig 2. IL-15 pretreatment reduces infiltration of eosinophils in Con A-induced liver injury.(A) Mice were injected with anti-mouse-IL-4 and -IL-5 Abs or control-IgG. Sera and livers were collected 16 h after Con A injection. ALT levels and EPO activities were measured. Values are shown as means &&num;x00b1;SEM (n=3). ** P<0.01. (B) Mice were sacrificed 1 h after Con A injection. RNA samples from Purified NKT cells or liver tissue were used to test expressions of IL-4, IL-5, eotaxin-1, eotaxin-2 and RANTES. RT-PCR analysis was used with corresponding primers. (C) Mice were treated with PBS or IL-15 6 h before Con A injection. Livers were collected 16 h after Con A injection. EPO activities were measured. (D) Liver samples were collected 16 h after Con A injection for anti-CCR3 staining (original magnification ? 200). N indicated necrosis area. Ten fields were randomly selected and the numbers of eosinophil were counted.

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