SEARCH

SEARCH BY CITATION

Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).

FilenameFormatSizeDescription
jws-hep.21212.fig1.tif68K Supplemental Figure 1 Atp8b1 deficiency does not affect Abcb11 and Abcg5 expression levels in the liver. Western blot analysis of liver plasma membrane preparation for Atp8b1, Abcb11, Abcg5, and Atp1a1. Atp1a1 was included as a protein loading control. Membrane preparations were pooled from n = 11 livers forAtp8b1 G308V/G308V mutant mice and n= 12 livers for wild-type mice.
jws-hep.21212.fig2.tif401K Supplementary Figure 2 Freeze-fracture electron microscopy (EM) shows the presence of multilamellar lipid structures. (A) Freeze-fracture EM image of wild-type liver (of mice fed a control diet) showing amorphous content of the canalicular lumen. Arrows indicate the base of a microvillus; bar, 1µm.(B) Freeze-fracture EM image ofAtp8b1 G308V/G308V mutantliver (of mice fed a control diet) showing a canalicular lumen filled withmultilamellar lipid (arrow); bar, 1µm.
jws-hep.21212.fig3.tif309K Supplemental Figure 3 Atp8b1 deficiency does not affect the localization of Abcb11 in liver as shown by Confocal Laser Scanning Microscopy (CLSM). (A) CLSM image of wild-type liver stained for Abcb11. (B) CLSM image ofAtp8b1 G308V/G308V mutant liver stained for Abcb11. Nuclear (dapi) staining in blue; green represents canalicular membrane staining. Bar, 15 µm.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.