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Liver Biology and Pathobiology
Atp8b1 deficiency in mice reduces resistance of the canalicular membrane to hydrophobic bile salts and impairs bile salt transport†
Article first published online: 23 JUN 2006
Copyright © 2006 American Association for the Study of Liver Diseases
Volume 44, Issue 1, pages 195–204, July 2006
How to Cite
Paulusma, C. C., Groen, A., Kunne, C., Ho-Mok, K. S., Spijkerboer, A. L., Rudi de Waart, D., Hoek, F. J., Vreeling, H., Hoeben, K. A., van Marle, J., Pawlikowska, L., Bull, L. N., Hofmann, A. F., Knisely, A. S. and Oude Elferink, R. P. J. (2006), Atp8b1 deficiency in mice reduces resistance of the canalicular membrane to hydrophobic bile salts and impairs bile salt transport. Hepatology, 44: 195–204. doi: 10.1002/hep.21212
Potential conflict of interest: Nothing to report.
- Issue published online: 23 JUN 2006
- Article first published online: 23 JUN 2006
- Manuscript Accepted: 29 MAR 2006
- Manuscript Received: 21 FEB 2006
- Netherlands Organization for Scientific Research. Grant Number: 912-02-073
- National Institutes of Health. Grant Number: R01 DK50697
Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21212.fig1.tif||68K||Supplemental Figure 1 Atp8b1 deficiency does not affect Abcb11 and Abcg5 expression levels in the liver. Western blot analysis of liver plasma membrane preparation for Atp8b1, Abcb11, Abcg5, and Atp1a1. Atp1a1 was included as a protein loading control. Membrane preparations were pooled from n = 11 livers forAtp8b1 G308V/G308V mutant mice and n= 12 livers for wild-type mice.|
|jws-hep.21212.fig2.tif||401K||Supplementary Figure 2 Freeze-fracture electron microscopy (EM) shows the presence of multilamellar lipid structures. (A) Freeze-fracture EM image of wild-type liver (of mice fed a control diet) showing amorphous content of the canalicular lumen. Arrows indicate the base of a microvillus; bar, 1µm.(B) Freeze-fracture EM image ofAtp8b1 G308V/G308V mutantliver (of mice fed a control diet) showing a canalicular lumen filled withmultilamellar lipid (arrow); bar, 1µm.|
|jws-hep.21212.fig3.tif||309K||Supplemental Figure 3 Atp8b1 deficiency does not affect the localization of Abcb11 in liver as shown by Confocal Laser Scanning Microscopy (CLSM). (A) CLSM image of wild-type liver stained for Abcb11. (B) CLSM image ofAtp8b1 G308V/G308V mutant liver stained for Abcb11. Nuclear (dapi) staining in blue; green represents canalicular membrane staining. Bar, 15 µm.|
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