Supplementary material for this article can be found on the H EPATOLOGY website ( ).

jws-hep.21212.fig1.tif68K Supplemental Figure 1 Atp8b1 deficiency does not affect Abcb11 and Abcg5 expression levels in the liver. Western blot analysis of liver plasma membrane preparation for Atp8b1, Abcb11, Abcg5, and Atp1a1. Atp1a1 was included as a protein loading control. Membrane preparations were pooled from n = 11 livers forAtp8b1 G308V/G308V mutant mice and n= 12 livers for wild-type mice.
jws-hep.21212.fig2.tif401K Supplementary Figure 2 Freeze-fracture electron microscopy (EM) shows the presence of multilamellar lipid structures. (A) Freeze-fracture EM image of wild-type liver (of mice fed a control diet) showing amorphous content of the canalicular lumen. Arrows indicate the base of a microvillus; bar, 1µm.(B) Freeze-fracture EM image ofAtp8b1 G308V/G308V mutantliver (of mice fed a control diet) showing a canalicular lumen filled withmultilamellar lipid (arrow); bar, 1µm.
jws-hep.21212.fig3.tif309K Supplemental Figure 3 Atp8b1 deficiency does not affect the localization of Abcb11 in liver as shown by Confocal Laser Scanning Microscopy (CLSM). (A) CLSM image of wild-type liver stained for Abcb11. (B) CLSM image ofAtp8b1 G308V/G308V mutant liver stained for Abcb11. Nuclear (dapi) staining in blue; green represents canalicular membrane staining. Bar, 15 µm.

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