TIP30 inhibits growth of HCC cell lines and inhibits HCC xenografts in mice in combination with 5-FU

Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html )

Filename	Format	Size	Description
jws-hep.21213.fig1.tif		4729K	Suppl Fig 1. TIP30 suppressed HCC cell invasion through ECM. HCC cells and human fibroblasts were incubated with ECM. Thirty-six (A) and 72 hours (B) latter, invasive cells were stained and counted. Data were presented as means?SD from three independent experiments. * P .05, **P .01.
jws-hep.21213.fig2.eps		1242K	Suppl Fig 2. TIP30-induced apoptosis was partly through p53. (A) HepG2 cells were stably transfected with pc-anti- p53or pcDNA3 vector. Total proteins were isolated and subjected to Western Blotting with anti-p53 antibody. (B) RNA was extracted from the same cells as above and subjected to real-time PCR analysis as described previously. (C) HepG2 cells stably expressing pc-anti- p53,pc-anti- GFPor pcDNA3 were infected with Ad-TIP30. The dead cells indicated by Trypan blue staining were counted under microscope at various times following infection. Data were presented as means?SD from three independent experiments. *P .01.
jws-hep.21213.fig3.tif		746K	Suppl Fig 3. Ad-TIP30 did not sensitize Chang liver cells to cytotoxic drug. (A) Chang liver cells were infected with Ad-TIP30 or Ad-GFP for 24 hours and then treated with etoposide at various concentrations for another 24 hours. Positive cells stained with Trypan blue were counted under microscope. Data were present as means?SD from three independent experiments. (B) Chang liver cells were stably transfected with anti-senseTip30or pcDNA3. Total proteins were isolated and subjected to Western Blotting with anti-TIP30 antibody. (C) Chang liver cells stably transfected with anti-senseTip30or pcDNA3 were treated with etoposide at various concentrations. The positive cells stained with Trypan blue were counted under microscope. Data were present as means?SD from three independent experiments. (D) Chang liver cells stably transfected with anti-senseTip30or pcDNA3 were infected with Ad-TIP30 or Ad-GFP. Total proteins were extracted and subjected to Western Blotting with anti-TIP30 antibody. (E) Chang liver cells stably transfected with anti-senseTip30were infected with Ad-TIP30 or Ad-GFP for 24 hours and then treated with etoposide at various concentrations. Trypan blue staining positive cells were counted under microscope. Data were present as means?SD from three independent experiments.*P .01.

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