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Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).

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jws-hep.21254.fig1.tif1378K Supplementary Fig. 1: Effect of LPS concentration on the synthesis of NO, IL-6 and TNF-&&num;x03b1;.Cells were washed and placed in serum-free medium containing indicated concentrations of LPS. At 24 hours, the medium was aspirated and analyzed for (A) NO and (B) IL-6 and TNF-&&num;x03b1; as described in the Methods section. Results are expressed as means &&num;x00b1; S.D. of triplicate determinations from a representative experiment. *p<0.05 vs "0"; **p<0.001 vs "0".
jws-hep.21254.fig2.tif750K Supplementary Fig. 2: Concentration-dependence of LPS-induced activation MAPKs.Cells were washed and placed in serum-free medium containing indicated concentrations of LPS. At 45 min of stimulation, cellular protein was extracted, subjected to electrophoresis and Western blotting was performed for phosphorylated (A) ERK1/2, (B) p38 and (C) JNK as described in the Methods section. Nonphosphorylated MAPKs were determined in the same membranes after stripping. The membranes were also stripped to determine actin expression to ascertain equal loading.
jws-hep.21254.fig3.tif357K Supplementary Fig. 3: Effect of H 2 O 2on the synthesis of NO, IL-6 and TNF-&&num;x03b1;.Cells were washed and placed in serum-free medium containing 100 &&num;x03bc;M H 2 O 2 . After 24 hours, the culture medium was analyzed for (A) NO and (B) IL-6 or TNF-&&num;x03b1;. Values are means &&num;x00b1; S.D. of triplicate determinations from a representative experiment. *p<0.001 vs control; **p<0.05 vs control.
jws-hep.21254.fig4.tif433K Supplementary Fig. 4: Time-course and concentration-dependence of H 2 O 2 ?induced TNF-&&num;x03b1; synthesis.Cells were washed and placed in serum-free medium containing (A) 100 &&num;x03bc;M H 2 O 2for indicated time periods or (B) indicated concentrations of H 2 O 2for 24 hours. The culture medium was analyzed for TNF-&&num;x03b1;. Values are means &&num;x00b1; S.D. of triplicate determinations from a representative experiment. (A) *p<0.05 vs 3 hour time point; **p<0.01 vs 3 hour time point; #p<0.05 vs control. (B) ***p<0.05 vs "0".

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