Liver Biology and Pathobiology

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Ischemic preconditioning of the murine liver protects through the Akt kinase pathway†

  1. Kunihiko Izuishi1,‡,*,
  2. Allan Tsung2,
  3. Mohammad Akram Hossain1,
  4. Masao Fujiwara1,
  5. Hisao Wakabayashi1,
  6. Tsutomu Masaki3,
  7. Timothy R. Billiar2,
  8. Hajime Maeta1

Article first published online: 29 AUG 2006

DOI: 10.1002/hep.21298

Issue

Hepatology

Hepatology

Volume 44, Issue 3, pages 573–580, September 2006

Additional Information

How to Cite

Izuishi, K., Tsung, A., Hossain, M. A., Fujiwara, M., Wakabayashi, H., Masaki, T., Billiar, T. R. and Maeta, H. (2006), Ischemic preconditioning of the murine liver protects through the Akt kinase pathway. Hepatology, 44: 573–580. doi: 10.1002/hep.21298

Author Information

  1. 1

    First Department of Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan

  2. 2

    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania

  3. 3

    Third Department of Internal Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan

  1. ‡

    fax: (81) 87-891-2187

*First Department of Surgery, Faculty of Medicine, Kagawa University, 1750-1, Miki, Kita, Kagawa 761-0793, Japan

  1. †

    Potential conflict of interest: Nothing to report.

  2. ‡

    fax: (81) 87-891-2187

Publication History

  1. Issue published online: 29 AUG 2006
  2. Article first published online: 29 AUG 2006
  3. Manuscript Accepted: 12 JUN 2006
  4. Manuscript Received: 12 DEC 2005

Funded by

  • Ministry of Education, Science, and Culture of Japan. Grant Number: Grant-in-Aid 14571202
  • National Institutes of Health. Grant Numbers: R01-GM50441, P50-GM53789

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FilenameFormatSizeDescription
jws-hep.21298.fig1.tif1766K Experimental protocol for ischemic preconditioning (IPC).Animals were subjected to 90 min ischemia followed by 180 min of reperfusion. IPC was induced by 10 min of ischemia followed by 10 min of reperfusion. Liver samples were obtained at the indicated time points.
jws-hep.21298.fig2.tif3826K Time course of Akt phosphorylation during ischemia and after reperfusion.Akt activation was examined in livers of animals undergoing various times of ischemia and reperfusion. Western blot analysis for phosphorylated Akt (A) and total Akt (B) levels was performed. Hepatic protein lysate from ischemic lobes were obtained. Blot shown is representative of three experiments with similar results. Akt phosphorylation was abolished by treatment with the PI3-kinase inhibitors wortmannin (W).

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