fax: (33) 1-40-61-30-12
The protein kinase IKKε can inhibit HCV expression independently of IFN and its own expression is downregulated in HCV-infected livers†
Article first published online: 28 NOV 2006
Copyright © 2006 American Association for the Study of Liver Diseases
Volume 44, Issue 6, pages 1635–1647, December 2006
How to Cite
Vilasco, M., Larrea, E., Vitour, D., Dabo, S., Breiman, A., Regnault, B., Riezu, J.-I., Eid, P., Prieto, J. and Meurs, E. F. (2006), The protein kinase IKKε can inhibit HCV expression independently of IFN and its own expression is downregulated in HCV-infected livers. Hepatology, 44: 1635–1647. doi: 10.1002/hep.21432
Potential conflict of interest: Nothing to report.
- Issue published online: 28 NOV 2006
- Article first published online: 28 NOV 2006
- Manuscript Accepted: 20 AUG 2006
- Manuscript Received: 5 MAY 2006
- Pasteur Institute. Grant Number: PTR#186
- Agence Nationale pour la Recherche contre le SIDA (ANRS)
- UTE project CIMA
Supplementary material for this article can be found on the H EPATOLOGY website ( http:// interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).
|jws-hep.21432.fig1.pdf||26K||Supplementary Fig. 1. Determination of the optimal concentrations of anti-IFNAR1 antibody on the HCV replicon Huh-7 cells for inhibition of IFN action Huh-7 cells expressing a full length HCV replicon were plated in 24-well plates at 1.5x105 cells/well and transfected after 24h with 1 ?g of pISRE-luciferase expressing vector (Stratagene). Three hours after transfection, different concentrations of anti-IFNAR1 were added in the media before addition of 10U/ml or 100U/ml of IFN after 45 minutes as indicated. Analysis of luciferase expression was performed after 24 hours as described in Materials and Methods.|
|jws-hep.21432.tbl1.pdf||12K||Supplementary Table 1: Primers used for reverse transcription and Real-Time PCR Except for the HCV primers, all primers were designed using the software LC Probe design (Roche). S: sense, AS:antisense|
|jws-hep.21432.tbl2.pdf||11K||Supplementary Table 2: Complete transcriptome profile induced by IKK in the Huh7 Replicon cells using Affymetrix U133 2.0 chips. The accession number of the genes is given on the left, followed by the gene name. The numbers represent the fold change and the mean values (of triplicate samples) for IKK-transfected and control HCV replicon cells|
|jws-hep.21432.tbl3.pdf||12K||Supporting Information file jws-hep.21432.tbl3.pdf|
|jws-hep.21432.tbl4.pdf||12K||Supporting Information file jws-hep.21432.tbl4.pdf|
|jws-hep.21432.tbl5.pdf||14K||Supporting Information file jws-hep.21432.tbl5.pdf|
|jws-hep.21432.tbl6.pdf||18K||Supporting Information file jws-hep.21432.tbl6.pdf|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.