Keratin 18 overexpression but not phosphorylation or filament organization blocks mouse Mallory body formation

Authors

  • Masaru Harada,

    1. Department of Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, and Stanford University Digestive Disease Center, Stanford, CA
    2. Department of Medicine, Kurume University School of Medicine, Kurume, Japan
    3. Stanford University Digestive Disease Center, Stanford, CA
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  • Pavel Strnad,

    1. Department of Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, and Stanford University Digestive Disease Center, Stanford, CA
    2. Stanford University Digestive Disease Center, Stanford, CA
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  • Evelyn Z. Resurreccion,

    1. Department of Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, and Stanford University Digestive Disease Center, Stanford, CA
    2. Stanford University Digestive Disease Center, Stanford, CA
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  • Nam-On Ku,

    1. Department of Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, and Stanford University Digestive Disease Center, Stanford, CA
    2. Stanford University Digestive Disease Center, Stanford, CA
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  • M. Bishr Omary

    Corresponding author
    1. Department of Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, and Stanford University Digestive Disease Center, Stanford, CA
    2. Stanford University Digestive Disease Center, Stanford, CA
    • Palo Alto VA Health Care System, 3801 Miranda Avenue, Mail code 154J, Palo Alto, CA 94304
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  • Potential conflict of interest: Nothing to report.

Abstract

Several human liver diseases are associated with formation of Mallory body (MB) inclusions. These hepatocyte cytoplasmic deposits are composed primarily of hyperphosphorylated keratins 8 and 18 (K8/K18). Feeding a 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-containing diet is a well-established mouse model of MBs. K8 overexpression, and K8-null or K18-null mouse models, indicate that a K8-greater-than-K18 expression ratio is critical for MB formation. We used established transgenic mouse models to study the effect of K18 overexpression and phosphorylation, or keratin filament disorganization, on MB formation. Five mouse lines were used: nontransgenic, those that overexpress wild-type K18 or the K18 phosphorylation mutants Ser33-to-Ala (S33A) or Ser52-to-Ala (S52A), and mice that overexpress K18 Arg89-to-Cys, which causes collapse of the keratin filament network into dots. DDC feeding induced MBs in nontransgenic livers, but MBs were rarely seen in any of the K18 transgenic mice. Wild-type K18 overexpression protected mice from DDC-induced liver injury. Conclusion: K18 overexpression protects mice from MB formation and from DDC-induced liver injury, which supports the importance of the K8-to-K18 ratio in MB formation. The effect of K18 on MB formation is independent of hepatocyte keratin filament organization or K18 Ser33/Ser52 phosphorylation. Keratin filament collapse, which is a major risk for acute liver injury, is well tolerated in the context of chronic DDC-mediated liver injury. HEPATOLOGY 2007;45:88–96.)

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