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jws-hep.21495.fig1.pdf306KSupplementary Fig. 1: Histological analysis of the ischemic area of a mouse liver 2 days after PHx (A)H&E-stained section of the ischemic area of the liver of aCasp8 F+: :Alb-Cremouse 2 days after PHx. A large focus of ghost cells and necrosis (N) of the hepatic parenchyma can be seen in the operated area. Suture material (S) is seen within the necrotic area. The necrotic parenchyma is surrounded by a rim of infiltrating leukocytes (I).(B)TUNEL staining of the area shown in the inset from (A), demonstrating massive apoptosis in this area (red staining indicates TUNEL-positive nuclei in the wounded liver parenchyma). Above this region are dead and ghost cells (no staining), while below there are living cells with DAPI-stained nuclei.(C)Anti active caspase-3 immunostaining of hepatocytes located at the rim of the wounded hepatic parenchyma. Both the cytoplasm and the nuclei are stained. Magnification: A, ×20; B, ×100; C: ×400.
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