Prevention of hepatocyte allograft rejection in rats by transferring adenoviral early region 3 genes into donor cells

Authors

  • Elena V. Mashalova,

    1. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY
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  • Chandan Guha,

    1. Department of Radiation Oncology, Albert Einstein College of Medicine, Bronx, NY
    2. Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY
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  • Namita Roy-Chowdhury,

    1. Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY
    2. Department of Medicine, Albert Einstein College of Medicine, Bronx, NY
    3. Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY
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  • Laibin Liu,

    1. Department of Radiation Oncology, Albert Einstein College of Medicine, Bronx, NY
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  • Ira J. Fox,

    1. Department of Surgery, University of Nebraska Medical Center, Omaha, NE
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  • Jayanta Roy-Chowdhury,

    Corresponding author
    1. Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY
    2. Department of Medicine, Albert Einstein College of Medicine, Bronx, NY
    3. Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY
    • Marion Bessin Liver Research Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461
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    • fax: 718-430-8975

  • Marshall S. Horwitz

    1. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY
    2. Department of Pediatrics, Albert Einstein College of Medicine, Bronx, NY
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    • Deceased.


  • This research was conducted by E. V. Mashalova in partial fulfillment of the requirements for the degree of doctor of philosophy at the Sue Golding Graduate Division of Medical Sciences, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY.

  • Potential conflict of interest: Nothing to report.

Abstract

Hepatocyte transplantation is being evaluated as an alternative to liver transplantation for metabolic support during liver failure and for definitive treatment of inherited liver diseases. However, as with liver transplantation, transplantation of allogeneic hepatocytes requires prolonged immunosuppression with its associated untoward effects. Therefore, we explored strategies for the genetic modification of donor hepatocytes that could eliminate allograft rejection, obviating the need for immunosuppression. Products of early region 3 (AdE3) of the adenoviral genome are known to protect infected cells from immune recognition and destruction. In the present study we showed that immortalized rat hepatocytes that had been stably transduced with AdE3 before transplantation into fully MHC-mismatched rats are protected from allograft rejection. Quantitative real-time PCR analysis showed that a similar number of engrafted AdE3-transfected hepatocytes had survived in syngeneic and allogeneic recipients. AdE3 expression did not reduce expression of MHC class I on the surfaces of donor hepatocytes. Consistent with this, the in vivo cytotoxic cell–mediated alloresponse was attenuated but not abolished in recipients of AdE3-transfected allogeneic hepatocytes. In contrast, graft survival correlated with a marked reduction in cell-surface localization of Fas receptor in the transplanted cells and inhibition of Fas-mediated apoptosis, which are related to the antiapoptotic functions of the AdE3 proteins. CONCLUSION:AdE3 gene products prevent hepatocyte allograft rejection mainly by protecting the cells from the effector limb of the host immune response and could be used as a tool to facilitate allogeneic hepatocyte transplantation. (HEPATOLOGY 2007;45:755–766.)

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