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Supplementary material for this article can be found on the H EPATOLOGY website ( http://interscience.wiley.com/jpages/0270-9139/suppmat/index.html ).

FilenameFormatSizeDescription
jws-hep.21529.fig1.pdf39K Supplemental Figure 1 - Schematic drawing of occult HBV full-length PCR amplification. The two subgenomic fragments, ?Fragment A? and ?Fragment B?, were amplified by nested PCR with two different combinations of primers and ligated by using the AvrII unique restriction site of HBV (nucleotide position 179).
jws-hep.21529.fig2.pdf9K Supplemental Figure 2 - Schematic representation showing mutations affecting the preS1 promoter in the representative HBV isolates of the 17 studied patients. Nucleotide coordinates for the genotype D are provided. Binding sites for the liver-specific transcription factors HNF-1 and HNF-3 and sites for interaction with TBP and Sp1 are indicated.
jws-hep.21529.fig3.pdf10K Supplemental Figure 3 - Schematic representation of mutations observed in the S promoter of the representative HBV isolates from the 17 studied patients. Numbering is according to genotype D. The NF-Y motif and sites for interaction with transcription factors NF1 and SP1 are shown.
jws-hep.21529.fig4.pdf57K Supplemental Figure 4 - Schematic representation of mutations observed in the enhancer I/X promoter complex of the representative HBV isolates form the 17 studied patients. Numbering is according to genotype D. Sites for interaction with specific transcription factors are indicated.
jws-hep.21529.fig5.pdf14K Supplemental Figure 5 - Schematic representation of mutations observed in the core promoter/enhancer II complex of the representative HBV isolates form the 17 studied patients. Numbering is according to genotype D. The TATA box-like sequences and transcription initiation sites of the precore and pregenomic RNA are indicated. Transcription factor binding sites are indicated as a schematic footprint.
jws-hep.21529.tbl1.pdf69K Supplementary Table 1 . Oligonucleotides used as PCR and Sequencing Primers
jws-hep.21529.pdf109KSupporting Information file jws-hep.21529.pdf

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